Sources of the Burkholderiales parB genes

ParB genes from Burkholderia cenocepacia J2315 (DSMZ), Burkholderia vietnamiensis G4 (J. Tiedje, Michigan State University), Ralstonia pickettii 12D and Ralstonia pickettii 12J (T. Marsh, Michigan State University) were amplified by PCR (Phusion High-Fidelity DNA Polymerase, Thermofisher Scientific) on genomic DNA extracted from these species.

ParB cloning and extract preparation

ParB extracts were prepared as described previously [26]. Briefly, wild-type parB genes cloned from start to stop codon with a consensus Shine-Dalgarno sequence were inserted under the control of the arabinose inducible pBAD promoter into the pDAG127 vector [30] with a deletion of the sopA gene by NheI/HindIII or EcoRI/HindIII digestion. Recombinant plasmids were electroporated into the Escherichia coli strain DLT812 [31]. Cells grown in LB medium at 37°C to an optical density at 600 nm (OD₆₀₀) of 0.25 were induced with 0.1% arabinose (final concentration), incubated for 4 h at 37°C, and chilled on ice for 10 min. Subsequent steps were carried out at 4°C. Cells were centrifuged, washed with TNE (50 mM Tris-HCl [pH 7.5], 50 mM NaCl, 1 mM EDTA) and resuspended in TNE at an OD₆₀₀ of 300. Lysozyme was added at 400 μg/ml; cells were then incubated for 5 min, frozen with liquid nitrogen, and thawed on ice. Lysis buffer (TNE, 660 mM NaCl, 4.5 mM dithiothreitol) was added to bring the OD₆₀₀ to 200. The mixture was incubated for 10 min on ice, and the cells were lysed by sonication. The lysate was centrifuged for 15 min at 10,600 × g and the supernatant centrifuged at 20,800 × g. The final supernatant was frozen with liquid nitrogen and stored at—20°C. Total protein concentrations were measured using a Bradford protein assay (Bio-Rad). Samples containing 3.5 μg of protein were electrophoresed in 4 to 12% poly-acrylamide bis-tris denaturing gels (NuPage; Invitrogen) at a constant voltage of 200 V for 55 min and then stained with a Coomassie blue derivative (InstantBlue Expedeon). Lanes containing no protein or protein from cells without ParB were also included on gels, and stained gel images captured with a gel-imager (Syngene G box) as.tif files were scanned and processed using Multi Gauge (Fuji) to quantitate roughly ParB as detailed previously [26]. The concentrations of ParB were estimated to range between 0.4 μg/ml (ParBpBC) and 2.8 μg/ml (ParBc1).

ParS probes and spotting conditions

All parS probes were designed as previously described [9], using 57-mer oligonucleotides modified at their 5’-ends with a thiol function (Sigma Aldrich, France). They consist of two inverted repeats of 26 bases–including the 16 bases parS or derivative sequences—separated by a five base spacer (see Fig 2A). Each oligonucleotide, resuspended at 3 μM in 3X SSC buffer containing 450 mM NaCl and 45 mM sodium citrate pH 7 (Sigma Aldrich, France) were spotted (25μM) on SPRi-Biochips covered with a 50 nm gold film using a ChipWriterPro contact spotter (Biorad) with solid pin SSP015 from Arrayit Corporation. For each probe, four spots were deposited in tandem at two different locations on the prism (Fig 2B).

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Fig 2. Experimental design.

(A) Structures of the 5’-thiol-labelled 57-mer single strand oligonucleotides carrying carrying parS, exemplified for parSc1. The parS sequence forms by the annealing of two inverted repeats of 26 bases (arrowed boxes). The upper structure shows the wild-type (wt) 16 bp palindrome of parSc1 by two arrowed black lines and with bp positions numbered. Three sequences modified from parSc1 are indicated below: randomly modified (rdm), one arm modified (half), and the double symmetric change T1C-A16G, (changed sequence is written in red) (B) Typical SPRi image captured at the end of injection (240 sec) of 10μg/ml of crude extract enriched in ParB c1. Each DNA probes was distributed in duplicate in two different localizations, as shown by white rectangles with parS wild type sequence (c1 wt) and white dotted rectangles with randomized sequence (c1 rdm). (C) Examples of kinetics curves for the six ParB-parS systems. For each ParB, the reflectivity variations according to time were observed with the wild-type parS (green curves) and the random parS (red curves). Various concentrations of crude extracts were injected: 10 (dotted lines), 20 (dashed lines) 40 μg/ml (full lines) for c1; 20 (dotted lines), 40 (dashed lines) and 80 μg/ml (full lines) for pBC, c3, 12D, 12J and G4.

https://doi.org/10.1371/journal.pone.0177056.g002

SPRi assays

SPRi experiments were performed using a SPRi-Plex system (GenOptics HORIBA-scientific, France) as described [32] using a running buffer (20 mM HEPES pH 7.4, 100 mM KCl, 0.1% bovine serum albumin—BSA, 50μg.ml⁻¹ sonicated salmon sperm DNA) at a flow rate of 50μl.min⁻¹. 200μl of ParB over-expressing crude cell extracts were injected during 240 s on the prism surface saturated with Denhardt solution (Sigma). The variation of reflectivity (VR) vs. time for each spot was analysed with Genoptics software SPRiAnalysis1.2.1. Mean values and standard deviations were directly calculated with this software from four independent spots. Between each injection, the regeneration of the prism surface was performed with NaOH 100 mM. The level of significance relative to the negative control (a sequence modified randomly relative to parS and indicated “rdm”) was determined by the student T test in excel software. Levels of significance are indicated in the “Sign.” columns of Figs A-G in S1 File as follows: * (P value < 0.05), ** (P value < 0.01), *** (P value <0.001). In addition a crude extract non over-producing ParB (referred to as ParB- extract) was also injected. For most probes the values obtained with this extract allow to discard the possibility that E. coli proteins contribute to the reflectivity. For the few probes that seemed to interact with proteins of the ParB- extract, we considered by default the VR obtained with ParB as non-significant—e.g. probes S160 and S161 (Fig F in S1 File), and probe S142 (Fig G in S1 File).

Setting up the SPRi conditions to determine ParB/parS interactions

The goal of this study was to analyse the interaction of six ParB proteins originating from six Burkholderiales replicons (chromosome, secondary chromosome or plasmid) with their cognate parS and many derivatives, in order to get insights on the specificities and similarities of these Par systems. Surface Plasmon Resonance imaging (SPRi) is adapted for such a wide analysis. Oligonucleotides allowing the formation of parS or derived sequences (exemplified in Fig 2A) are spotted on the same prism. ParB is injected and the variation of reflectivity was measured at each oligonuclotide spot (Fig 2B). Crude Escherichia coli extracts over-expressing one ParB were used in this study, as they are at least as sensitive as purified ParB proteins in detecting DNA probes in SPRi assays [9]. Three protein concentrations were used: 10, 20 and 40 μg/ml for ParBc1; 20, 40 and 80 μg/ml for the 5 other ParBs. Upon injection of ParB, the variation of reflectivity (VR) for each ParB increases with time up to a maximum value, indicating that equilibrium between association and dissociation is reached. For each ParB, the VR obtained with the cognate parS is above that obtained with the randomly changed parS (Fig 2C, compare green and red curves for the same protein concentrations) indicating that the specific binding for cognate parS is detected. Increasing the protein concentrations leads to globally increased curves of VR, suggesting the used concentrations are not saturating, and thus valid to determine ParB-parS interactions. In some cases the VR decreases before the end of injection (240 s), which nevertheless suggests a mass transport due to excess proteins. This is particularly noticeable for ParBc1 at the highest concentration (Fig 2C). For ParB12D there is a time delay for detecting the increase of VR, which also occurs at the highest protein concentration. To correlate thereafter the VR and the interaction of ParBs with modified parSs, we thus kept the VR obtained with the smallest (10 μg/ml for ParBc1) or the intermediate (40 μg/ml for other ParBs) protein concentrations. For each ParB / parS interaction tested, we defined a binding score (BS) by taking the VR value at the end of injection. Many probes with four or more changes on the full parS length have been tested, giving mostly VR close to non specific, so that we will not discuss these results in details. They indicate roughly that parS sequences cannot be changed drastically to maintain a well detected ParB/parS interaction by SPRi. The results that we present and discuss are those relative to the probes designed to (i) characterize the binding of ParB to one arm of parS and (ii) determine the importance of each position in parS for binding ParB.

The sequences of all tested probes, the BS obtained with extracts over-producing ParB (ParB+) and extracts non-producing ParB (ParB-), the standard deviations and the significance of the values are shown in S1 File.

Mutating one arm of the palindromic parSs

With the exception of parS-12J, the studied parSs are perfect palindromes of 16 bp (Fig 1). They consist of two contiguous inverted repeats (IR) of 8 bp, referred to as parS arms. ParBs of the present study have a helix-turn-helix (HTH) domain previously identified [17, 26], so that each monomer of the ParB dimer could interact with one arm of parS, as it is the case for the ParB protein of the F plasmid [8, 9]. Some probes designed with one nucleotide change in just one arm of parS-c1, -c3, and -pBC, gave high BS, similar to those of the wt controls (T1G, T1A, T3C, T3A, T5A in parSc1;G1T, G1C, C6T in parSc3; C1G, C6A, C6T, T7G in parSpBC–Figs A-C in S1 File). However these probes do not allow determine if the remaining normal arm is responsible for the high BS or if the nucleotide change impairs poorly the interaction. To convincingly determine if one arm maintains binding to ParB we designed oligonucleotide probes, called “half-parS”, with one arm randomly changed (Figs A-G in S1 File, and Fig 2A for c1).

For c1, c3, and pBC (Fig 3A, 3B and 3C) the BSs of half-parS (orange lines) are intermediate i.e. below the specific BS (wt, green lines) and significantly above the non-specific BS (rdm, red lines). Thus, in these cases one arm of parS retains specific binding for ParB, as described the centromere of the F plasmid—which is composed of two inverted repeats separated by two bases [9]. Disparities nevertheless appear between the BS induced by these three half-parSs: that of pBC allows a very high BS, just below the wt parS pBC, while half-parSc1 and half-parSc3 generate weaker BS relative to their wt parS.

For 12D and G4, the BSs of half-parSs are just above and at the non-specific value respectively (Fig 3D and 3E). We cannot formally exclude that SPRi could not discriminate between reduced and non-specific interactions due to a lack of sensitivity. But this seems unlikely because with the same ParB extracts (12D and G4) tested on other derivatives of parSG4, SPRi was able to reproducibly measure weak BSs, just above the non-specific value (Fig 3D and 3E). If there is a lack of sensitivity of the SPRi, it does not question the fact that the interaction of ParB12D and ParBG4 with half-parSG4 is weak, close to the interaction observed with non-specific DNA.

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Fig 3. Binding scores for palindromic parS and derivatives.

(A) ParB c1 B. cenocepacia; (B) ParB c3 B. cenocepacia; (C) ParB plasmid pBC B. cenocepacia; (D) ParB plasmid 1 Ralstonia picketti 12D; (E) ParB plasmid 2 Burkholderia vietnamiensis G4. Binding scores correspond to 100-fold the VR value at the end of the SPRi injection. The average scores for the wild-type (wt) and random (rdm) sequence are represented as lines, green and red respectively. Average scores for the half-parS sequences are shown as orange lines. For double symmetric substitutions, the score is directly pointed on the graph as the base substituting the wild-type one indicated below on the x-axis. Changes are indicated for one arm only but correspond to symmetrical changes (e.g the change T1C in parSc1 means T1C and A16G). For each change statistical analysis of the BS were performed; the substitutions displaying a significantly different BS relative to that of non specific DNA are wrote in red.

https://doi.org/10.1371/journal.pone.0177056.g003

Double symmetrical mutations in palindromic parSs

To determine the relative importance of each nucleotide at a given position of the five palindromic centromeres we designed probes mutated at each pair of symmetrical positions (i.e 1–16, 2–15, 3–14, 4–13, 5–12, 6–11, 7–10 and 8–9) by two or three complementary substitutions. These probes were assayed by SPRi (Fig 3A–3E).

For the five palindromic parS the distal position (1–16) allows the largest amount of substitutions while maintaining a BS well above that of the non-specific DNA, even reaching or exceeding the BS of the wt parS in the case of c3. Some changes, internal to the parSs, also maintain a BS well above the non-specific. This is notably the case at positions (2–15) and (6–11) in parSc3, (5–12) in parSc1, (7–10) in parSpBC and (5–12) in parSG4. For these internal positions the highest BSs correspond in general to transitions, e.g. T5C in parSc1, C5T in parSG4, C6T in parSc3 and T7C in parSpBC. The highest BS for internal transversions is T2A in parSc3.

Interestingly, in parSc1 the BS is completely dropped to basic by all changes (Fig 3A), with the exceptions of positions (1–16) and (5–12) already mentionned. Such a narrow specificity of each position is less obvious in the other parSs, notably parSc3 (Fig 3B).

Changes in the non palindromic parS12J

The sequence parS12J is repeated five times upstream the corresponding parAB operon on a plasmid integrated into the chromosome of Ralstonia pickettii 12J [26]. The arms of parS12J are not strictly complementary, one being identical to parSc3 and the second differing at two positions (Fig 1); they will be referred to as “parSc3” and “non-parSc3” arms, respectively. The interaction of ParB12J with parS12J and its derivative probes were assayed by SPRi (Fig 4). The reference BSs were 54 for the cognate parS 12J (wt, green line in Fig 4A and 4B) and 11 for the non-specific DNA (rdm, red lines in Fig 4A and 4B). The BS of ParB12J for “parSc3” and “non-parSc3” full palindromes are high (69 and 41 respectively; Fig 4A and 4B blue lines). Thus ParB12J has the capability to bind palindromic parSs, and the parSc3 palindrome appears a better substrate than the “non parSc3” palindrome but also better than its cognate parS12J. We therefore tested the interaction of ParB12J with two “half-parSs” sequences keeping either the “parSc3” or the “non-parSc3” arm. They displayed a BS of 25 and 14 respectively (orange lines; Fig 4A and 4B), confirming that parSc3 is a better substrate than non-parSc3, but mainly suggesting that both arms of parS are important for the binding ParB12J, as observed notably for ParB12D and ParBG4.

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Fig 4. Binding scores of ParB12 J for parSc3 non-parSc3 sequences and derivatives.

Blue lines correspond to the BS of the palindromic sequence parSc3 (A) and non parSc3 (B). Legend is the same as in Fig 3.

https://doi.org/10.1371/journal.pone.0177056.g004

We then analyzed the effect on the BS of double symmetric changes introduced in full palindromes, either “parSc3” or “non-parSc3” (Fig 4A and 4B). In both palindromes changes at the distal position (1–16) strongly impair ParB binding. This result indicates that for ParB12J the distal position of the palindrome is important for the specificity of interaction, in contrast to all the other parSs. Few internal positions accept changes while maintaining a significant BS with ParB12J: position (5–12) in the “parSc3” palindrome; positions (2–15) and (4–13) in the “non-parSc3” palindrome. Interestingly, in the “non-parSc3” palindrome the best accepted changes are transversions: T2A and T4G (Fig 4B).

Validation of the SPRi technology by comparing to previous techniques

The SPRi multiplex technology allows the analysis of multiple ParB/parS interactions simultaneously. This is an advantage over the techniques that we previously used in the same goal, i.e. electrophoretic mobility shift assay (EMSA) and an in vivo test of incompatibility [26]. Briefly, for the in vivo test, B. cenocepacia was transformed with plasmids carrying extra-parS sequences; a growth delay of transformants indicated the extra-parS bind ParB while a normal growth indicated the extra-parS is impaired for binding ParB. Interestingly, the interaction with ParB of some parSc1, parSc3 and parS12J derivatives tested here by SPRi, was also tested by incompatibility and (or) EMSA, allowing to discuss the reliability of SPRi.

It appears that the three techniques (in vivo growth delay, EMSA, and SPRi) give their highest responses for the wild-type parSs. In addition, all the mutations that maintained a delayed growth in vivo, display a high SPRi BS. It is the case for single-arm mutations T1G, T1A of parSc1, and G1T, G1C, C6T of parSc3 (Figures A-B in S1 File, and [26]). It is also the case for the double symmetrical mutations T1G-A16C and T5C-A12G of parSc1 (Fig 3A, Figure A in S1 File, and [26]). Conversely all changes leading to a low BS did not delay growth in vivo: double symmetrical A7T-T10A in parSc1; T5G-A12C and A7T-T10A in parSc3 (Fig 3A and 3B; Figures A- B in S1 File, and [26]). Also, we found that the changes that maintain a relatively high BS in this study, led to a band shift in EMSA: e.g single-arm T1G and T1A in parSc1; double symmetrical T1G-A16C, T5C-A12G in parSc1, and double symmetrical C6T-G11A in parSc3.

We nevertheless observe that few parS derivatives that maintain a relatively high BS did not delay growth by incompatibility, e.g single arm mutations T3C, T3A, T4A and T5A, in parSc1 (Figure A in S1 File and [26]). However, these derivatives are subtly modified parSs (one nucleotide change on one arm), so that the detection of their interaction with ParB could be particularly dependent on the technique used. For example in vivo, any binding defect of the extra-parS should be aggravated by its competition with the genomic parS, rendering the in vivo approach more stringent but less suitable than SPRi to reveal weak interactions between ParBs and modified parS.

Alltogether, these data suggest that, for most parS, especially those wild-type and their multi-mutated derivatives, SPRi compares with the other techniques. Besides, it was established for the ParB protein of the F plasmid that a two-fold reduction of the reflectivity–measured by SPRi for a half centromere site—corresponded to a 100 fold loss of affinity [9]. Certainly this observation indicates that the reflectivity is not proportional to the affinity; on the other hand it suggests that most ParB/parS interactions, even those lessened compared to the wt, are readily detected by SPRi. Thus SPRi is a valuable tool for the rough and wide detection of ParB/parS interactions.

Influence of the symmetrical structure of parS for binding ParB

ParBc1, ParBc3 and ParBpBC display for their half-parS a BS significantly above the negative control value: 49%, 77% and 88% respectively of the BS for wild-type parS. This behavior is shared by the ParB protein of the F plasmid [9]; and ParB2 of Vibrio cholerae was found to bind a non centromeric site provided this site contains a half-parS sequence [33]. This suggests that for many ParBs the initial interaction may occur at one arm of parS only. For ParBpBC the reduction of BS is low (12%) which might further suggests that once a monomer has interacted with the normal arm, the second monomer binds DNA whatever the sequence. At the opposite, the BS for half-parS was severely impaired for ParB12D and dropped to basic for ParBG4. In the latter case the binding of each HTH to both parS arms seems necessary for the stable interaction of ParB.

ParB12J, whose natural parS is not symmetrical—one arm like parSc3 and one arm “non parSc3” differing at two positions, also displays the capability to bind just one arm. The parSc3 arm is clearly the favorite (Fig 4A and 4B orange lines). More interestingly, we found that ParB12J interacts with both palindromes corresponding to the perfect inverted repeat of each arm of parS12J, i.e. parSc3 and “non parSc3” palindromes (Fig 4A and 4B blue lines). The BS of ParB12J was even higher with parSc3 than with its own parS12J centromere. However, the non symmetrical structure of parS12J is repeated identically on DNA and maintained in two replicons (in Ralstonia pickettii 12J on a plasmid integrated into the chromosome, and in R.pickettii 12D on a free plasmid). The conservation of this asymmetry suggests that a specific adaption of ParB to this centromere occured, but certainly not for optimizing its binding. The formation of a partition complex depends on the affinity of ParB for its minimal parS target but also on the number of repeats of this target, the further spreading of ParB etc… Improving the function of a Par system might sometimes be achieved through reducing the ParB/parS affinity.

Determination of the positions tolerant to changes

Position (1–16) of parSc1, c3, pBC, 12D and G4 undergoes nucleotide changes while maintaining the interaction with the cognate ParB. This has already been reported [9,26,34] and suggests that the distal position has less importance for the specific binding of ParB. An exception appears: ParB12J, whose binding to the palindromes parSc3 and “non-parSc3” is abolished when they are doubly modified at this position. If, as discussed above, ParB specifically adapted to a non-symmetrical parS, this adaptation might, on the other hand, limit the spectrum of the parS modifications maintaining an interaction with ParB. In the context of “not natural” centromeres (i.e. palindromic in this case), the distal position (1–16) could be more crucial for the binding.

In most parSs we found some internal positions also permissive to changes, like position (5–12) in parSc1, parSG4 and parSc3 (in the latter case for binding ParB12J), position (6–11) in parSc3 for binding ParBc3, and position (7–10) in parSpBC (Figs 3 and 4). It is also the case of the position (2–15) in parSc3 for binding ParBc3, and in the “non-parSc3” palindrome for binding ParB12J. Interestingly in both cases the transversion T2A is the best tolerated change, which is atypical—we found mostly transitions in the less destabilizing changes, and strengthens the kinship previously proposed between 12J and c3. The patterns of permissive nucleotide changes thus appear partly shared by few parS and partly specific.

The specific case of parSc1

ParSc1 appears the less permissive centromere to nucleotide changes: apart those distal (1–16) and the transition at (5–12), all symmetrical changes abolished the interaction (Fig 3A). This narrow specificity for binding ParB could be linked to the multi-chromosomal environment of Burkholderia: a nucleotide change allowing ParB of another chromosome to bind parS of the major chromosome would be detrimental for the genome stability. As parSc1 has the canonical sequence of all parSs of single-chromosome genomes, it is interesting to determine if parSc1 is less permissive than parS of single chromosomes. The analysis of nucleotide changes accepted in chromosomal parS have been carried out in Bacillus subtilis [15,35], Streptomyces coelicolor [36], Pseudomonas aeruginosa [34] and the main chromosome of Vibrio cholerae—which has two chromosomes [37]. Analysis were carried out by chromatin immunoprecipitation (ChIP), EMSA or fluorescence microscopy, testing only native parS variants, so that they are not as wide as our SPRi analysis. Nevertheless a single change at only one distal position and one single transition at position 5 or 12 (not the double change) naturally occurs in all parS and maintains an interaction with ParB, but less efficiently in chromosome 1 of Vibrio cholerae. To a lower extent, positions 7 or 10 appear permissive in B. subtilis, P. aeruginosa and S. coelicolor. By contrast in V. cholerae, like in B. cenocepacia, none of these latter positions are permissive to mutations. We cannot exclude from these results that the interaction between ParB and parS of the main chromosome is indeed more constrained in multi-chromosomal genomes.

As indicated, the single transition at position 5 or 12 naturally occurs on chromosomal parS, and the double transition at 5–12 maintains a high BS in our analysis. This well tolerated change could be finally fixed in some bacteria, allowing the emergence of a variant form of parS. In this direction, it is interesting to point out that, while in most species the canonical parS is TGTTTCACGTGAAACA—with only few repeats degenerated at position 5 or 1, all chromosomal parSs of Pseudomonas species share the motif TGTTCCACGTGGAACA [17,34], which is precisely changed at the position (5–12)—underlined. In Pseudomonas species the double symmetrical transition T5C-A12G certainly took over the original parS because of an undetermined selective pressure with a probable co-adaptation of ParB.

Drawing evolving links between the other Par systems of B. cenocepacia

In parSc3, at least three positions (1–16, 2–15 and 6–11) are tolerant to changes (Fig 3B). We previously proposed that c3 has kept the features of an ancestral Par system from which 12D, 12J and G4 have emerged. The permissiveness of parSc3 to nucleotide changes strengthens this idea: like the position (5–12) of c1 discussed above, any nucleotide change accepted in the ancestral parSc3 might be fixed, evolving progressively a new parS sequence. The change G1A-C16T well accepted by parSc3, might have contributed to the formation of parSG4; the tolerance of parSc3 to the change C6A-G11T is less obvious—Fig 4B and Figure B in S1 File, but cannot be excluded. Further adapting modifications of ParBs would have allowed the emergence of new but related Par systems forming the Bsr3 family.

Beyond the evolutionary changes proposed within the Bsr3 family, other evolving links between the Par systems of B. cenocepacia could be considered, for example between pBC and c3. We recently found that the segregation of the origin of c3 is essentially mediated by its Par system, is less controlled by the cell cycle than those of c1 and c2, and is mostly similar to the segregation of pBC [38]. Thus c3 of B. cenocepacia could have a plasmid ancestor and this plasmid could be that of B. cenocepacia i.e. pBC. In this hypothesis, parSc3 might have formed from parSpBC. Three double changes are required in parSpBC for such a transformation: C1G-G16C, G5T-C12A and T7A-A10T. SPRi shows that the first change is very well accepted by parSpBC, but the two other ones much less (Fig 3C and Figure C in S1 File). Nevertheless among internal positions of parSpBC, (5–12) and (7–10) appear weakly tolerant to some changes. Besides once a mutation is fixed in a parS sequence, it generates a new parS motif, which should influence both an adaptation of ParB and the further capacity of parS to change again. That parSc3 form from parSpBC, even-though progressively, thus remains an attractive possibility.