http://orcid.org/0000-0003-0797-269X
Figures
Abstract
An ongoing study of the genus Merodon Meigen, 1803 in the Republic of South Africa (RSA) has revealed the existence of new species related to M. melanocerus Bezzi, 1915. The M. melanocerus subgroup belongs to the Afrotropical lineage of the M. desuturinus group. Revision of all available material from museums and detailed analyses of newly -collected specimens from our own expeditions to RSA resulted in delimitation of five species: M. capensis Hurkmans sp. n., M. commutabilis Radenković et Vujić sp. n., M. drakonis Vujić et Radenković sp. n., M. flavocerus Hurkmans sp. n. and M. melanocerus. In addition to classical morphological characters, sequences of the mitochondrial COI gene are provided for four related taxa. Results of molecular phylogenetic analyses supports monophyly of the M. desuturinus group and confirmed delimitation between species. Links between Palaearctic and Afrotropical faunas of this group, as well as possible evolutionary paths, are discussed. Based on phylogenetic analyses, four lineages (putative subgenera) have been recognized within the genus Merodon; besides the three previously established ones, albifrons+desuturinus, aureus (sensu lato) and avidus-nigritarsis, one new lineage named natans is distinguished.
Citation: Radenković S, Veličković N, Ssymank A, Obreht Vidaković D, Djan M, Ståhls G, et al. (2018) Close relatives of Mediterranean endemo-relict hoverflies (Diptera, Syrphidae) in South Africa: Morphological and molecular evidence in the Merodon melanocerus subgroup. PLoS ONE 13(7): e0200805. https://doi.org/10.1371/journal.pone.0200805
Editor: Oliver Schweiger, Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Umwelt und Gesundheit, GERMANY
Received: July 21, 2017; Accepted: June 29, 2018; Published: July 20, 2018
Copyright: © 2018 Radenković et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: Data are available in the Supporting Information files: S1 Table lists all specimens used for genetic analyses and sequences are submitted in GenBank and codes are provided.
Funding: This work was funded by a project of the European Union: Horizon 2020, Marie Skłodowska-Curie action, Research and Innovation Staff Exchange (RISE) Programme: FlyHigh - Insect-plant relationships: insights into biodiversity and new applications (project number 645636, 2015-2018); and partially by the RSA SAEON network, a member of International Long-Term Ecological Research Network (ILTER) and Ministry of Education, Science and Technological Development of the Republic of Serbia (OI173002 to AV and III43002).
Competing interests: The authors have declared that no competing interests exist.
Introduction
The phytophagous hoverfly genus Merodon Meigen (Diptera: Syrphidae: Eristalinae: Merodontini) is distributed over the Palaearctic and Ethiopian regions and comprises more than 160 species [1]. The highest diversity of this genus is recorded in the Mediterranean region, which has been associated with the high variety of bulb plants that are larval hosts [2, 3, 4]. The species that has received the most attention is the narcissus bulb fly, Merodon equestris Fabricius, 1794, which is considered a pest in flower bulb cultivation. This is the only representative of the genus in the Nearctic region, where it was probably inadvertently introduced.
The most comprehensive revision of the Merodon genus was done by Hurkmans [5], who assessed 61 species and classified them into 11 groups (alagoezicus, alexeji, avidus, clavipes, crassifemoris, elegans, longicornis, nigritarsis, pruni, tarsatus and vandergooti) in the first part of the monograph. The second part of the monograph has never been published, but handed to Ante Vujić in manuscript form. Later, a series of papers dealing with particular groups of species, such as aureus, nanus, nigritarsis and ruficornis, have appeared [6–12]. The Merodon fauna of the Balkan Peninsula, Aegean Islands, Turkey and the Iberian Peninsula are the most comprehensively explored in Europe [1, 13–21], contrasting sharply with the hoverfly fauna of the Afrotropical region. Although this region harbours about 600 hoverfly species, representatives of the genus Merodon are very rare. Less than ten species have been recorded, and most data are from South Africa [22]. Intriguingly, the Cape region is the most diverse in bulb plants of the African continent, with ca. 1200 species [23].
The Merodon desuturinus species group is very interesting because it represents an important link between the Palaearctic and Afrotropical faunas, and may reveal the possible origin of the genus. Vujić, Šimić and Radenković [24] described the Balkan endemic Merodon desuturinus Vujić, Šimić et Radenković, 1995 from high mountains and, later, Marcos-García et al. [14] discovered another related species, M. cabanerensis Marcos-García, Vujić et Mengual, 2007 from Spain that was also confirmed in Morocco [25]. Vujić et al. [25] described one additional species M. neolydicus Vujić, 2018, distributed in the Eastern Mediterranean, and redescribed M. murorum (Fabricius, 1794) from North-West Africa. These authors, as well as Milankov, Ståhls and Vujić [26], highlighted diagnostic characters for the desuturinus group and emphasized that the African species M. cuthbertsoni Curran, 1939 and M. planifacies Bezzi, 1915, also clearly belonged to this group.
A basic phylogeny of the genus was established by Mengual et al. [13] who defined four well-supported clades based on analysis of COI sequences from 17 Iberian species: the desuturinus, albifrons, nigritarsis (avidus) and aureus groups. Milankov et al. [26] analysed genetic relationships among populations of M. desuturinus and taxa from the aureus, avidus and ruficornis groups from the Balkan Peninsula and observed low genetic variability in populations of M. desuturinus. Furthermore, Milankov et al. [26] showed that M. desuturinus represents an evolutionarily independent lineage among Merodon taxa and that an integrative taxonomic approach should be applied for further insights into the evolutionary history and phylogenetic position of the M. desuturinus group. Šašić et al. [12] merged the albifrons and desuturinus groups into one clade according to the results of Vujić et al. [9]. This is in agreement with close relationships between these two groups previously described by Milankov et al. [26], who also highlighted the ruficornis group (belonging to the albifrons clade) as being the closest one to desuturinus.
A recent barcoding study of Afrotropical hoverflies [27] stressed the importance of using molecular data to assist morphological identification of species and ecological studies, since identification of Afrotropical hoverflies is challenging. Nuclear rDNA (ITS2, 28S, 18S) and mtDNA sequences are commonly used genetic markers in population genetic studies, taxonomy, systematics and conservation genetics. The mtDNA cytochrome c oxidase I (COI) gene has repeatedly been used as a useful taxonomic tool for species delimitation in the family Syrphidae [1, 9, 12, 12, 19, 20, 28]. Integrative taxonomic studies have used both the 3’- and 5’-ends of COI. The latter is widely regarded as a “barcoding” sequence and it represents one of the most used genetic markers for species identification [29–33]. The 3’-end of COI also constitutes a useful taxonomic tool in studies of the Syrphidae [9, 34, 35]. According to Jordaens et al. [27], there are few COI barcodes of Afrotropical hoverflies in public databases (BOLD Identification System and GenBank). For South Africa, we found 23 records of hoverfly COI sequences available in GenBank (in a search of the nucleotide collection with keywords ‘Syrphidae’ and ‘South Africa’ on June 27th, 2018), with no representative of the genus Merodon. Jordaens et al. [27] provided a COI barcode reference database for 98 Afrotropical hoverfly species, mostly from western Africa, but did not include species from the genus Merodon. They encouraged expansion of the database to other genera and geographical areas since molecular data not only assist species identification, but also provide a basis to pinpoint taxa that need further taxonomic studies, help identify recent introductions, and can be useful for linking conspecific males and females having discordant morphologies and larvae with adults, thereby enhancing our understanding of the ecology of particular species.
Specifically, here we present an ongoing study of the genus Merodon in the Republic of South Africa (RSA), as well as a detailed analysis of available material from museums and private collections, that has revealed the existence of new species of the desuturinus group, related to M. melanocerus Bezzi, 1915, native to southeastern RSA. In addition to classical morphological characters, we obtained sequences of the 3’ and 5’ regions of the mitochondrial COI gene for 23 specimens of the M. melanocerus subgroup and combined the data with that from 27 other Merodon taxa. Records noted here represent the first detailed characterisation of Merodon species in South Africa based on classical morphological and molecular data, and constitute a contribution towards a molecular database of Afrotropical Syrphidae.
Material and methods
Morphological analysis
Permits for insect collecting were obtained from Ezemwelo KwaZulu-Natal province: permits OP 3754/2015 and OP 4603/2016, Eastern Cape province: CRO 15/16CR and CRO 02/16CR. This revision is based on the examination of Merodon specimens collected in RSA during expeditions between 2011 and 2016 [collectors: Jelena Ačanski (BioSense, Serbia), Celeste Pérez Bañón (CEUA, Spain), Ximo Mengual (ZFMK, Germany), Marija Miličić (FSUNS, Serbia), Snežana Radenković (FSUNS, Serbia), Santos Rojo (CEUA, Spain), Axel Ssymank (A.S.coll., Germany), Gunilla https://www.researchgate.net/profile/Gunilla_StahlsStåhls (MZH, Finland), Nevena Veličković (FSUNS, Serbia) and Ante Vujić (FSUNS, Serbia). In addition, we studied all the available Afrotropical material previously cited in the bibliography and unpublished material deposited in the museums, universities and private collections listed below. Type specimens of all Merodon species described from the Afrotropics were also studied. The following acronyms for museums and entomological collections containing studied material are used in the text: BMNH—Natural History Museum, London, UK; CEUA—Colección Entomológica Universidad de Alicante, Spain; FSUNS—Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Serbia; MZH—Finnish Museum of Natural History, University of Helsinki, Finland; NMNL—National Museum of Natural History Naturalis, Leiden, Netherlands; NMSA—Natal Museum, Pietermaritzburg, Republic of South Africa; ZFMK—Zoological Research Museum Alexander Koenig, Bonn, Germany; ZMUC—Zoological Museum, University of Copenhagen, Denmark; A.S.coll.—Axel Ssymank collection, Germany; M.B.coll.—Miroslav Bartak collection, Czech Republic, M.R.coll.—Menno Reemer collection, Netherlands. Data on the geographic distribution of analyzed species were processed in DivaGis (v7.5) [36] and presented on Fig 1. The characters used follow the terminology established by Thompson [37], Doczkal and Pape [38] and those relating to male genitalia are those employed by Marcos-García et al. [14]. Colour characters are described from dry-mounted specimens. The male genitalia were extracted from dry specimens previously relaxed in a closed pot with a high level of humidity, by using an insect pin with a hooked tip. They were cleared by boiling in warm 10% potassium hydroxide (KOH) for 3–5 minutes. Acetic acid was then used to neutralize the KOH during 5 seconds. Genitalia were stored in microvials containing glycerol. Specimen measurements were taken in dorsal view with a micrometer and are presented as ranges. Body length was measured from the lunula to the end of the abdomen, and wing length from the base of the tegula to the wing apex. Drawings were made with an FSA 25 PE drawing tube attached to a binocular microscope Leica MZ16. Photos were made with a Leica DFC320 camera connected to a personal computer. After photographing, CombineZ software [39] was used in order to create composite image with an extended depth of field, created from the in- focus areas of each image. Photo of pile was taken with a JEOL JSM 6460LV scanning electron microscope (SEM) operated at 20 kV.
A short description is provided for each new species, including figures of adult morphology. Diagnoses comprise accounts of unique characters relative to the group, lineage, subgroup and species considered here, and also combinations of characters that enable taxa to be distinguished and recognized. Keys are also provided to enable identification of adults. The type material was examined by Ante Vujić.
Nomenclatural acts
The electronic edition of this article conforms to the requirements of the amended International Code of Zoological Nomenclature, and hence the new names contained herein are available under that Code from the electronic edition of this article. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix “http://zoobank.org/”. The LSID for this publication is: urn:lsid:zoobank.org:pub: 9DD25199-47F6-412E-B89A-D4A1D9505161. The electronic edition of this work was published in a journal with an ISSN, and has been archived and is available from the following digital repositories: PubMed Central, LOCKSS.
Molecular analysis
DNA extraction.
Total genomic DNA was extracted using 1 to 3 legs from 23 dry, pinned specimens of the M. melanocerus subgroup following the procedure described by Chen et al. [40]. Genomic DNA vouchers were accordingly labelled and conserved at the Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad (FSUNS).
PCR amplification and sequencing.
The mtDNA COI 3’ fragments were amplified using the forward primer C1-J-2183 (5’-CAA CAT TTATTT TGA TTT TTT GG-3’) (alias JERRY) and reverse primer TL2-N-3014 (5’-TCC AAT GCA CTA ATC TGC CAT ATT 3’) (alias PAT) [41]. Additionally, the Folmer fragment or ‘barcode fragment’ of the 5' region of COI was amplified using standard polymerase chain reaction protocols with the forward primer LCO (5'-GCTCAACAAATCATAAAGATATTGG-3') and reverse primer HCO (5'-TAAACTTCAGGGTGACCAAAAAATCA-3') [42]. The reaction mix contained 1x reaction buffer (Thermo Scientific), 2.5 mM MgCl2, 0.1 mM of each nucleotide, 2 pmol of each primer, 1U Taq polymerase (Thermo Scientific), and approximately 50 ng template DNA in a total volume of 25μl. Amplification was performed using the following conditions: 95°C for 2 min; 29 cycles of 94°C for 30s each, 49°C (for 3’ COI) and 50°C (for 5’ COI) for 30 s; 72°C for 2 min; with the final extension at 72°C for 8 min. The PCR products were purified using ExoSAP (Thermo Scientific) following the manufacturer’s recommendations. Sequencing was conducted on an ABI3730xl Genetic Analyzer (Applied Biosystems, Folmer City, CA, USA).
Phylogenetic analysis.
Obtained sequences of the 3’ and 5’ regions of COI were aligned using the Clustal W algorithm [43] implemented in BioEdit [44], and final adjustments were performed manually. In order to establish the systematic position of the M. melanocerus subgroup, our phylogenetic analyses also included several species belonging to the M. desuturinus group, Merodon species from the Palaearctic region representing the main lineages following Mengual et al. [13], and six species as outgroups (see S1 Table for accession numbers of examined species and outgroups).
For construction of phylogenetic trees, we removed identical sequences using DAMBE v.5 [45]. The Maximum Likelihood (ML) tree was constructed using MEGA 7 [46] under the general time-reversible (GTR) evolutionary model using a discrete Gamma distribution with five rate categories and by assuming that a certain fraction of sites are evolutionarily invariable as it was shown as the best evolutionary model for the generated dataset (as estimated in MEGA 7). Branch support was estimated with 100 non-parametric bootstrap replicates. Bayesian phylogenetic analyses (BI) were also carried out using the same evolutionary model as for ML tree, as priors in MrBayes ver.3.2 [47]. Two independent runs of four Markov chain Monte Carlo (MCMC) permutations were performed for 20,000,000 generations with sampling every 100 generations. Tracer v1.5 [48] was used to summarise the Bayesian analysis and to inspect the validity of the burn-in fraction applied. The first 25% of the sampled iterations/generations were discarded as burn-in, and 50% consensus trees were computed using FigTree v1.4.0 [49]. Additionally, a phylogenetic analysis, as inferred by Maximum Parsimony (MP) using NONA [50], was conducted with the aid of Winclada [51] using the heuristic search algorithm with 1000 random addition replicates (mult*1000), holding 100 trees per round (hold/100), max trees set to 100 000 and applying tree-bisection-reconnection (TBR) branch swapping. Nodal support for the resulting consensus tree was assessed using non-parametric bootstrapping with 1000 replicates. The tree was rooted with Microdon bidens.
Results
Systematic position and diagnostic characters of the Merodon melanocerus subgroup
The Merodon melanocerus subgroup, defined here based on morphological characteristics, belongs to the M. desuturinus group sensu Mengual et al. [13] and is closely related to the albifrons group [13]. The M. desuturinus group is characterized by the following adult morphological characters: posterior surface of mesocoxa with pile; anterior lobe of surstyle of male genitalia with curved distal prolongation (as in Fig 2B: a); and the specific shape of the lateral sclerite of the aedeagus (which is the main synapomorphic character for the group) (as in Fig 3D: l) [24, 25, 26]. This group contains a Palaearctic and an Afrotropical lineages. The main morphological diagnostic character that separates these two lineages is the presence of a dense and strong yellow-to-red brush of pile on the metatrochanter in Afrotropical species, which is lacking in Palaearctic taxa. In addition to M. desuturinus, the Palaearctic lineage includes three more species: M. cabanerensis, M. murorum and M. neolydicus. The Afrotropical lineage comprises four known taxa: M. cuthbertsoni, M. melanocerus, M. planifacies and M. stevensoni Curran, 1939. New studies have revealed cryptic species within the taxon M. planifacies and new species related to M. melanocerus. The M. planifacies and the M. stevensoni share a clear apomorphic character, i.e., a reduced oral margin covered by microtrichia (Fig 4A and 4B) and together represent a subgroup named M. planifacies [25]. Of the remaining species in the group, M. cuthbertsoni is very close to M. desuturinus according to morphological characters, and treated together with Mediterranean representatives of the group [25]. Looking at distributions, members of the M. planifacies subgroup are endemics distributed in Western, Central and Southern Africa, contrary to the species of the melanocerus group whose ranges are restricted to Southern Africa. The species M. cuthbertsoni is endemic, only known from Zimbabwe.
(A) epandrium, lateral view; (B) surstyle lobe, ventral view; (C) hypandrium, lateral view; (D) apical part of the hypandrium, ventral view; (a) anterior lobe of surstyle; (t) inner thorn on median part of surstyle; (p) posterior lobe of surstyle; (c) cercus; (l) lateral sclerite of aedeagus; (r) ventral ridge of theca.
(A) epandrium, lateral view; (B) surstyle lobe, ventral view; (C) hypandrium, lateral view; (D) aedeagus with accessory structures, lateral view; (a) anterior lobe of surstyle; (at) inner thorn on anterior lobe of surstyle; (p) posterior lobe of surstyle; (t) inner thorns on median part of surstyle; (c) cercus; (l) lateral sclerite of aedeagus; (r) ventral ridge of theca.
(A, B) Merodon planifacies Bezzi, 1915 (holotype): (A) lateral view; (B) face, anterior view. (C) Merodon desuturinus Vujić, Šimić et Radenković, 1995 (Kopaonik, Serbia), lateral view.
Based on our results we provide a revision of taxa related to the species M. melanocerus that cluster together in a subgroup of the M. desuturinus group, all having the following additional diagnostic characters: protruded and shiny oral margin and face; thorax black (except in M. flavocerus sp. n. with orange postpronotum and postalar calli); tergite 2 often with a pair of orange lateral spots (except in M. capensis sp. n. and M. commutabilis sp. n.); punctuation in general less dense, especially on lateral sides of tergites; females can be distinguished from related species by long and outstanding white pile on sternites and on the lateral sides of tergites, especially when compared with the length of adpressed pile on the central parts of the tergites.
General description of Merodon melanocerus subgroup
Male. Body pile generally branched (Fig 5). Head (as in Fig 6A): Antenna (as in Fig 6C) usually dark brown; basoflagellomere generally short, as long as broad (except in M. flavocerus sp. n. where it is light brown and longer, Fig 7D), concave dorsally, with acute apex; arista light brown to dark brown, thickened basally, 1.5–2 times longer than basoflagellomere, covered with dense brown microtrichia. Face shiny black, with narrow white microtrichose stripe along eye margin, often missing in lower third (except in M. flavocerus, where it is present all along eye margin), covered with long whitish-yellow pile, except on median bare stripe that occupies 1/4 width of face. Oral margin shiny black, well protruded (as in Figs 6A and 7A). Frons black, often with bronze shine and indistinct microtrichia that, at level of face, run in a narrow line along the eye margin. Vertical triangle isosceles (as in Fig 7C), black (brown-red in M. flavocerus), shiny except for anterior end with microtrichia; predominantly covered with long, black, thick pile (very thick in M. commutabilis sp. n. and M. drakonis sp. n.), except at posterior end with pale yellow pile (in M. melanocerus sp. n. pale pile also present on anterior end). Eye pile dense, long as scape, often pale yellow, but can be darker dorsally (in M. commutabilis black and thick dorsally). Occiput covered with whitish-yellow pile; dorsally with metallic, bluish or bronze lustre; white microtrichia start from upper eye corner as a narrow line dorsally, becoming dense and wide laterally and ventrally, occupying lower 2/3 of occiput.
(A) head of male, lateral view: (o) oral margin; (B) head of female, lateral view; (C) antenna of male, lateral view; (D) antenna of female, lateral view; (E) hind leg of male, anterior view; (F) hind leg of female, anterior view.
(A) head of male, lateral view: (o) oral margin; (B) head of female, lateral view; (C) head of male, dorsal view; (D) antenna of male, lateral view: (f) fossette; (E) antenna of female, lateral view.
Thorax: Scutum and scutellum black with bronze lustre (in M. flavocerus, postpronotum and posterior rim of scutellum pale yellow); presence of microtrichia variable (from well-developed in M. drakonis to absent in M. capensis); covered with relatively long (as long as, or a little longer than basoflagellomere), dense, erect, more or less branched, usually yellow pile (in M. capensis and M. commutabilis mixed with black pile). Pleurae often covered with gray-green microtrichia (lacking in M. flavocerus) and the following parts with long yellow pile: anterior part of proepimeron, posterior part of anterior anepisternum, most of the posterior anepisternum except anterior end, antero-ventral and postero-dorsal part of katepisternum, anepimeron, metasternum; katatergite with dense, erect, short, yellowish or light brown pile. Wing hyaline, with dense microtrichia and light brown to dark brown veins. Calypter yellow. Halter with brown pedicel and yellow to brown capitulum. Legs usually dark brown to black (light brown in M. flavocerus), except paler knees, and sometimes paler base and apex of tibiae (like in M. melanocerus and M. flavocerus); colour of tarsi varies. Metatrochanter without process, covered with brush of yellow-to-orange, dense, strong pile. Metafemur moderately thickened and straight or slightly curved. Metatibia with apical, inconspicuous antero-ventral spur and indication of postero-ventral spur. Pile on legs predominantly yellow, except some short black pile dorsally on tarsi (and sometimes in M. capensis and M. commutabilis partly present on femora).
Abdomen: Black with bronze reflections, slightly tapering, as long as mesonotum. Tergites 2–4 black with more or less distinct white transverse band of microtrichia interrupted in the middle (can be connected on tergite 4); tergite 2 with a pair of orange antero-lateral spots (lacking in M. capensis and M. commutabilis, which have dark tergites) or areas covered with long, dense, erect, yellow pile; pilosity on lateral sides of tergites long, erect and whitish, but adpressed on central parts, white on mictrotichose transversal bands, posterior 2/3 of tergite 4 and also on hind margin of tergites 2–3 of most species (except in M. capensis), otherwise black. Sternites shiny, dark brown (except yellow in M. flavocerus), covered with very long pale yellow pile.
Male genitalia: Posterior lobe of surstyle triangular, usually pointed apically (as in Fig 2A: p); ventral margin of anterior lobe of surstyle straight (as in Fig 3A: a) or convex (as in Fig 8A: a); anterior lobe of surstyle bent inwards (as in Fig 2B: a); median part of surstyle with one or two inner thorns (as in Fig 8B: t); cercus elongated (as in Figs 2A and 3A: c). Hypandrium with broad theca (as in Fig 2C). Lateral sclerite of aedeagus narrow, gradually tapering, with the tip curved downwards (as in Fig 3D: l).
(A) epandrium, lateral view; (B) surstyle lobe, ventral view; (C) hypandrium, lateral view; (D) apical part of the hypandrium, ventral view; (a) anterior lobe of surstyle; (p) posterior lobe of surstyle; (t) inner thorns on median part of surstyle; (c) cercus; (l) lateral sclerite of aedeagus; (s) subapical lamella of theca; (w) lateral wings of theca.
Female. Similar to the male except for normal sexual dimorphism (as in Fig 9D).
(A-F) Merodon melanocerus Bezzi, 1915 (RSA): (A) head of male, lateral view: (o) oral margin; (B) head of female, lateral view; (C) vertex of male, dorsal view; (D) head of female, dorsal view; (E) antenna of male, lateral view; (F) antenna of female, lateral view. (G) Merodon drakonis sp. n. (RSA, Drakensberg Mountains): vertex of female, dorsal view.
Length: medium-sized species, body 10–12 mm, wing 6–8 mm (n = 65).
Biology. Flight period is mainly from June to December, although some specimens were also recorded in February. Adults were observed resting on leaves of low vegetation (grass, bulb plants), and in some cases feeding on flowers of Senecio spp. Larvae are unknown.
Merodon capensis Hurkmans sp. n. urn:lsid:zoobank.org:act:D04600C6-D629-4456-B5F1-3986C2378983 (Figs 2 and 6).
Holotype. ♂, RSA, Northern Cape, Nieuwoudtville, Hantam National Botanical Garden, 31o 23' 51" S, 19o 08' 24" E, 11 September 2012, J. & A. Londt (sweep net), (NMSA) (GUN7 = 08899).
Paratypes: RSA, Cape. 30km NE Wellington, Bainskloof Pass, 1♀, 27 September 1979, J. Londt (sweep net) (ZMUC) (04330); RSA, Capland, Stellenbosch, 1♂, 25 December 1925, H. Brauns (sweep net), det. P.H. van Doesburg as Lampetia nasica Bezzi, (NMNL) (LML-05-6); RSA, Capland, Stellenbosch, 2mm, 25 September 1925, H. Brauns (sweep net), (NMSA); RSA, Capland, Stellenbosch, 1♂, 25 December 1925, H. Brauns (sweep net), det. P.H. van Doesburg as Lampetia nasica Bezzi, (NMSA)(48544); RSA, Northern Cape, Nieuwoudtville, Hantam National Botanical Garden, 31o 23' 51" S, 19o 08' 24" E, 1♂, 1♀, 11 September 2012, J. & A. Londt, (NMSA) (08898, 08900).
Range (Fig 1). Endemic species for the Cape Region in RSA. Flight period: from September to December.
Etymology. The name capensis is derived from the word ‘Cape’ referring to the Cape Province in South Africa, the type area of the species.
Diagnosis. Scutum between wing bases with stripe of black pile, usually without microtrichia (except in some specimens present on anterior margin, but without longitudinal microtrichose stripes or microtrichia on transverse suture). Тergites black; transversal stripes of microtrichia on tergites 2–4 narrow, less than 1/8 of their length, in some males can be absent on tergite 4. Tibiae and tarsi partly pale-yellow-brown, especially basal two or three tarsal segments on pro- and mesolegs. Thecal ridge folded in male genitalia (Fig 2C and 2D: r). Female frons mostly black pilose. According to structure of male genitalia and genetic data, the most closely related species is M. melanocerus (Fig 10), from which it can be easily distinguished by lack of orange lateral spots on tergite 2 and the absence of a large inner thorn on the anterior lobe of surstyle of the male genitalia, as well as the wider hypandrium, besides previously mentioned diagnostic characters. Most similar taxon is M. commutabilis, which is also a black species but has completely black legs (contrary to M. capensis) and a conspicuously narrower posterior lobe of surstyle of the male genitalia.
Numbers on nodes depict Bayesian posterior probabilities. The scale bar indicates expected number of changes by site. Four lineages observed in genus Merodon are marked on the tree. For species that belong to Merodon melanocerus subgroup, photos of adult specimens and male genitalia are presented.
Description. Male. Head (Fig 6A): Antenna (Fig 6C) brown-to-black, basoflagellomere 1.1–1.2 times as long as wide, 1.4 times longer than pedicel; arista dark brown, 1.5 times longer than basoflagellomere. Vertical triangle isosceles, twice as long as eye contiguity. Ocellar triangle equilateral. Eye contiguity about 10–12 ommatidia long. Eye pile mostly pale yellow.
Thorax: Scutum and scutellum black with bronze lustre (usually without microtrichia except in some specimens on anterior margin of scutum), covered with dense, erect, gray-whitish or yellow pile, except for stripe of black pile between wing bases. Wing hyaline with dark-brown veins, and dense microtrichia except for cell bc, and above and below spurious vein in proximal 2/3 of cell br, with reduced microtrichia. Femora and tibiae brown-black, except usually with paler knees and base of tibiae; tarsi dark brown dorsally (except usually pale on basal two or three tarsal segments on pro- and mesolegs), orange-to-light brown ventrally. Pile on legs yellow, but in some specimens antero-dorsal part of pro- and mesofemora and apical 1/3 of metafemur can have short black pile, besides to black pile on dorsal surface of metatarsus. Metafemur (Fig 6E) slightly curved.
Abdomen: Tergites 2–4 black with more or less distinct white transverse band of microtrichia interrupted in the middle (in some specimens lacking on tergite 4); pile on tergites erect and whitish-yellow on lateral sides, but tergites 2–4 medially with adpressed black pile, except white pile on microtrichose bands.
Male genitalia (Fig 2): Anterior lobe of surstyle bent inwards (Fig 2B: a), with ventral margin slightly convex (Fig 2A); median part of surstyle with one inner thorn (Fig 2B: t); posterior lobe of surstyle wide, triangular, pointed apically (Fig 2A: p). Hypandrium wide, with folded thecal ridge (Fig 2C and 2D: r).
Female. Similar to the male except for normal sexual dimorphism (Fig 6B, 6D and 6F) and shiny frons without microtrichia (exceptionally with very narrow line of microtrichia along eye margin), mostly covered with black pile.
Merodon commutabilis Radenković et Vujić sp. n. urn:lsid:zoobank.org:act:6707360D-736D-495A-8CAE-07F4C29EA65E (Fig 8).
Holotype. ♂, RSA, KwaZulu-Natal, Howick, near Currys Post, 29° 21' 43.9" S, 30° 5' 54.9" E, 18 October 2015, A. Vujić (sweep net), (NMSA) (ZA1_014).
Paratypes: RSA, KwaZulu-Natal, Howick, near Currys Post, 29° 21' 43.9" S, 30° 5' 54.9" E, 17♂♂, 3♀♀, 18 October 2015, A. Vujić, J. Ačanski & M. Miličić (sweep net), (CEUA, FSUNS, NMSA & MZH) (ZA1_013, ZA1_015–032, ZA1_215); RSA, KwaZulu-Natal, Van Reenen, 1♂, Drakensberg, December 1926, R. E. Turner (sweep net), (BMNH); RSA, KwaZulu-Natal, near Howick, meadow near exit 125 of N3 to Balgowan, 1260m, 29o 21' 45.9" S, 30o 05' 52.5" E, 2♂♂, 2♀♀, 18 October 2015, X. Mengual (sweep net), (ZFMK) (12193–12196).
Range (Fig 1). Distributed in KwaZulu-Natal Province in RSA. Flight period: October to December.
Etymology. The name commutabilis is a Latin word meaning changeable/variable, referring to the great variability of pile colour on the scutum.
Diagnosis. Black species with white narrow transversal microtrichose bands on tergites 2–4, similar to Merodon capensis sp. n., but clearly different in male genitalia structure (hypandrium with unfolded theca, but with distinct subapical lamellas and lateral wings; epandrium with much narrower posterior lobe of surstyle and more convex ventral margin of anterior lobe of surstyle), presence of microtrichose stripes on scutum (in M. capensis absent or reduced microtrichia on anterior margin), and black legs (partly pale in M. capensis). Based on the structure of the male genitalia and genetic data, it is closest to M. drakonis (Fig 10), from which it can be distinguished by the lack of large, distinct, orange lateral spots on tergite 2 and subtle differences in male genitalic characters (hypandrium and epandrium shorter, posterior lobe of surstyle wider). Body pile very thick and branched, especially visible on vertical triangle and scutum.
Description. Male. Head: Antenna dark-brown, basoflagellomere 1.1 times as long as wide, 1.2 times longer than pedicel, concave dorsally, apex acute; arista about twice as long as basoflagellomere. Vertical triangle isosceles, 2.5 times longer than eye contiguity, covered with long black pile. Ocellar triangle isosceles. Eye contiguity about 10 ommatidia long. Eye pile mostly pale yellow, except for thick black pile on dorsal 1/3.
Thorax: Scutum and scutellum black with bronze lustre, and three more or less developed white microtrichose longitudinal stripes (from distinct three stripes long as 2/3 length of scutum to specimens with indistinct medial stripe reaching level of transverse suture and reduced submedial stripes) and microtrichia on transverse suture; colour of pile on scutum extremely variable, from all pale, whitish or yellowish to specimens with a stripe of black pile between wing bases or almost completely black pilosee in some specimens. Wing hyaline, with dense microtrichia and brownish veins. Halter brown. Legs dark brown to black, exceptionally with paler ventral side of tarsi. Metatrochanter with very distinct brush of hairs. Legs predominantly covered with pale yellow pile, except short black pile on dorsal and antero-dorsal parts of pro- and mesofemora, and in some specimens also on distal end of metafemur. Metafemur slightly thickened and curved.
Abdomen: Tergites 2–4 black, with distinct white transverse band of microtrichia interrupted in the middle; tergite 2 usually completely black, exceptionally with small, vague, orange or brown spot in front of mictrotrichose band, never reaching lateral margin of tergite; pile on tergites mainly erect and yellow on lateral sides, but tergites 2–4 medially with black adpressed pile.
Male genitalia (Fig 8): Ventral margin of anterior lobe of surstyle convex (Fig 8A: a); median part of surstyle with two inner thorns (Fig 8B: t); posterior lobe of surstyle elongated, pointed apically (Fig 8A: p). Hypandrium broad, with smooth thecal ridge (Fig 8C), well-developed subapical lamellas (Fig 8C and 8D: s) and lateral wings (Fig 8C and 8D: w).
Female. Similar to the male except for normal sexual dimorphism and shiny frons with microtrichia only present along eye margin, covered with mixed pale yellow and black pile.
Merodon drakonis Vujić et Radenković sp. n. urn:lsid:zoobank.org:act:44EFC9F3-E610-4F60-A1E6-6AC3F0F75495 (Figs 5, 9G and 11).
Holotype. ♂, RSA, Royal Natal NP, Thendele, 28o 41' 18" S, 28o 55' 50.7" E, 1–6 December 2012, A. Vujić (sweep net), (NMSA) (G2137 = GUN15).
(A) epandrium, lateral view; (B) surstyle lobe, ventral view; (C) hypandrium, lateral view; (D) apical part of the hypandrium, ventral view; (a) anterior lobe of surstyle; (p) posterior lobe of surstyle; (t) inner thorns on median part of surstyle; (c) cercus; (l) lateral sclerite of aedeagus; (s) subapical lamella of theca; (w) lateral wings of theca.
Paratypes: RSA, Royal Natal NP, Thendele, 28o 41' 18" S, 28o 55' 50.7" E, 7♂♂, 2♀♀, 1–6 December 2012, C. Pérez-Bañón, G. Ståhls & A. Vujić (sweep net), (CEUA, FSUNS, NMSA & MZH) (G2133, G2135, AF55, G2174—G2178); Thendele, 28o 43' 0" S, 28o 55' 0" E, 1♂, 3 December 2012, S. Radenković (sweep net), (FSUNS) (G2125); RSA, Royal Natal NP, Crack, 28o 41' 18" S, 28o 55' 50.7" E, 3♂♂, 8–9 December 2012, S. Rojo (sweep net), (CEUA, FSUNS & NMSA) (05778, AF56, G2212); RSA, Royal Natal NP, Sentinel, 28° 44' 23.9"S, 28° 53' 2" E, 1♂, 2♀♀, 8 December 2012, A. Vujić (sweep net), (FSUNS) (G2222, AF60, G2227); RSA, Natal, Benvie farm, 29o 15' 30" S, 30o 20' 40" E, mixed Podocarpus forest edge, 1♂, 4 December 1987, J. Londt & A. Seymour (sweep net), (NMSA) (50843); RSA, Royal Natal NP, Gorge Car Park, 28o 42' 48.5" S, 28o 56' 06.0" E, 1530m, 2mm, 3 December 2012, A. Ssymank (sweep net), (A.S.coll.) (AB 10); RSA, Cathedral Peak, Rainbow Gorge, 1♂, November 2011, Vujić A. (sweep net), (FSUNS) (G0878); RSA, KwaZulu-Natal, near Howick, meadow near exit 125 of N3 to Balgowan, 1260m, 29o 21' 45.9" S, 30o 05' 52.5" E, 1♂, 18 October 2015, X. Mengual (sweep net), (ZFMK) (12184); RSA, Drakensberg Mountains, Sunday Falls, -28° 40' 16.3" S, 28° 57' 11" E, 11 ♂♂, 1♀, 22 February 2016, S. Radenković, N. Veličković & A. Vujić (sweep net), (NMSA & FSUNS) (ZA2_137- ZA2_148).
Range (Fig 1). Endemic species to Drakensberg Mountains in RSA. Sympatric and synchronic with M. commutabilis. Flight period: February and November/December.
Etymology. The name drakonis is derived from the name of the type area, i.e. Drakensberg ("mountains of dragons"), the main mountain range in South Africa.
Diagnosis. This species is very similar in appearance to Merodon melanocerus (short basoflagellomere, almost as long as wide; tergite 2 with reddish-orange lateral spots). The main differences between males of these two species are the shapes of the genitalia: posterior lobe of surstyle narrow, straight, pointed, with a small apical ridge (Fig 11A: p) (in M. melanocerus triangular, without apical ridge, Fig 3A: p); ventral margin of anterior lobe of surstyle convex (Fig 11A: a) (in M. melanocerus almost straight, Fig 3A: a). For females, the distance between the posterior ocellus and upper eye corner in M. drakonis sp. n. is less than the distance between ocelli (Fig 9G) (in M. melanocerus, this distance is larger, Fig 9D). Based on the structure of male genitalia and genetic data (Fig 10), it is most closely related to M. commutabilis (see diagnosis of M. commutabilis).
Description. Male. Head: Antenna dark-brown, basoflagellomere 1.1–1.2 times as long as wide, 1.1–1.3 times longer than pedicel; arista about twice as long as basoflagellomere. Vertical triangle isosceles, 2.5 times longer than eye contiguity. Ocellar triangle slightly isosceles. Eye contiguity about 10 ommatidia long. Eye pile mostly pale yellow, except for some black pile on apical 1/6.
Thorax: Scutum with well-developed whitish microtrichia: anteriorly, laterally on transverse suture and on intraalar area with longitudinal stripe extending from transverse suture to the level of posterior end of postalar callus, medially with three longitudinal stripes long as 2/3 length of scutum. Microtrichia of pleurae conspicuous, the most developed on katepisternum, gray-green in colour. Halter brown. Legs dark brown-black, except slightly paler on knees and ventral side of tarsi. Metafemur slightly thickened and curved. Legs with pale yellow pile.
Abdomen: Tergites 2–4 with distinct white transverse band of microtrichia interrupted in the middle; tergite 2 with large, orange antero-lateral spots; pile on tergites mainly erect and yellow on lateral sides, but tergites 2–4 medially with black adpressed pile. Sternites can be paler, especially posterior half of sternite 1, almost all of sternite 2 and partly sternite 3 light brown; shiny, except in some specimens the anterior margin of sternite 4 has microtrichia.
Male genitalia (Fig 11): Epandrium and hypandrium generally prolate (Fig 11A and 11C). Ventral margin of anterior lobe of surstyle convex (Fig 11A: a); posterior lobe of surstyle elongated, apically pointed, with small ridge (Fig 11A: p); median part of surstyle with two inner thorns (Fig 11B: t). Hypandrium with smooth thecal ridge (Fig 11C), distinct subapical lamellas (Fig 11C and 11D: s) and lateral wings (Fig 11C and 11D: w).
Female. Similar to the male except for normal sexual dimorphism (Fig 9G) and for the following characteristics: frons with transversal striae, shiny in the center; laterally, beside microtrichose line along eye margin, also with triangular microtrichose areas.
Merodon flavocerus Hurkmans sp. n. urn:lsid:zoobank.org:act:2BFDB16C-95AF-4B38-B07B-AA4AFF69343A (Figs 7, 12A and 12B).
Holotype. ♂, RSA, Cape Province, 15 km SE Kirkwood, 4 November 1978, R. Miller & J. Londt (sweep net), (NMSA).
Paratypes: RSA, George, Cape Colony, 1♂, 25 December 1922, 1♂, 25 September 1922, (NMNL), 1 October 1920, 1♀, (NMSA), Dr Brauns (sweep net), det. v. Doesburg as Lampetia melanocera Bezzi); RSA, Bontebok NP, Western Cape, 1♀, 13–15 October 1993, F. Koch (sweep net), (M.B.coll.); RSA, Pt. Elizabeth, 1♂, 28 February 1919, H. K. Munro (sweep net), det. v. Doesburg as Lampetia melanocera Bezzi, (NMSA) (50556); RSA, Cape Province, Kleinemonde, 1♂, 11 September 1959, D. J. Greathead (sweep net), (NMNL).
Range (Fig 1). Species restricted to Cape Province in RSA. Flight period: February and September to November.
Etymology. The epithet flavocerus had already been affixed to the holotype by F. M. Hull, who did not publish the species description; this epithet has been retained by Hurkmans in his unpublished manuscript. It was probably given by Hull in reference to the yellowish antennae. It is to be treated as a noun.
Diagnosis. Basoflagellomere, orange-brown, elongated 1.5–2.0 times as long as wide (Fig 7D and 7E); metafemur straight, not curved (Fig 12A and 12B); katepisternum shiny; tergites 3–4 with reddish transversal stripes. In males, eyes dichoptic (Fig 7C); posterior lobe of surstyle short, broad and triangular (Fig 13A: p).
(A, B) Merodon flavocerus sp. n. (paratype, RSA); (C, D) Merodon melanocerus Bezzi, 1915 (RSA); (A, C) male; (B, D) female.
Merodon flavocerus sp. n., male genitalia (paratype, RSA); (A) epandrium, lateral view; (B) surstyle lobe, ventral view; (C) hypandrium, lateral view; (D) apical part of the hypandrium, ventral view; (a) anterior lobe of surstyle; (p) posterior lobe of surstyle; (t) inner thorns on median part of surstyle; (c) cercus; (l) lateral sclerite of aedeagus; (r) ventral ridge of theca.
Description. Male. Head (Fig 7A and 7C): Antenna (Fig 7D) orange-brown, basoflagellomere 1.8–2.0 times as long as wide, 1.5 times longer than pedicel, with wide fossette occupying whole dorsal surface (Fig 7D: f); arista: second and third flagellomeres yellow, fourth flagellomere light brown basally and dark brown apically, 1.5 times longer than basoflagellomere. Face shiny black, except narrow white microtrichose line along entire eye margin. Vertical triangle (Fig 7C) light brown, shiny red-brown between posterior ocellus and upper eye corner, isosceles, covered with long and mainly black pile. Ocellar triangle (Fig 7C) equilateral. Eyes dichoptic. Eye pile as long as scape, pale yellow.
Thorax: Scutum and scutellum black with bronze lustre, except pale yellow postpronotum and posterior rim of scutellum, as well as light brown transverse suture and postalar callus; predominantly shiny, but pospronotum and adjacent anterior areas with white microtrichia. Pleurae shiny without microtrichia. Wing hyaline, with brown veins and dense, light brown microtrichia (except in proximal 2/3 of br cell and above spurious vein with reduced microtrichia). Halter light brown. Femora brown, except usually with paler apex; pro- and mesotibiae completely orange-brown or vague shading subapically, metatibia orange-brown basally and apically; colour of tarsi varies (usually pro- and mesotarsi with two apical tarsomeres darkened dorsally and metatarsus dorsally darkened, the rest orange-brown). Metafemur thickened and straight (Fig 12A). Legs with pale yellow pile, except for some short black pile dorsally on tarsi.
Abdomen: Tergites predominately brown; except orange-yellow lateral spots on tergite 2, lateral sides of tergites, transversal bands (can be indistinct on tergite 4) and posterior 1/4 of tergite 4; transversal bands usually covered with white microtrichia. Pilosity pale yellow laterally on tergites, also whole of tergite 1 and most of tergite 4, as well as on orange transversal bands; black short adpressed pile cover posterior half of tergite 2 and dark areas of tergite 3. Sternites yellowish, covered with long pale yellow pile.
Male genitalia (Fig 13): Anterior lobe of surstyle with convex ventral margin (Fig 13A: a); median part of surstyle with two inner thorns (Fig 13B: t); posterior lobe of surstyle broad, short and triangular (Fig 13A: p). Hypandrium narrow, with unfolded thecal ridge (Fig 13C: r).
Female. Similar to the male except for normal sexual dimorphism (Figs 7B, 7E and 12B) and for the following characteristics: basoflagellomere 1.5–1.7 times as long as wide, with small, narrow, furrow-like fossette; frons and vertex without microtrichia except for narrow line along eye margin.
Merodon melanocerus Bezzi, 1915 (Figs 3, 9A–9F, 12C and 12D).
Holotype. ♀, Merodon melanocerus Bezzi, 1915: 101, RSA, Piet Retief Transvaal, 25 September 1903, R. Crawshay (sweep net), (BMNH).
Other material: RSA, Port St. John, 1♂, October 1916, (NMSA), 1♂, (NMNL), H. H. Swiny (sweep net), det. v. Doesburg as Lampetia melanocera Bezzi, (08693); Port St. John, Pondoland, 1♀, 6–25 February 1924, R. E. Turner (sweep net), (BMNH) (specimen bore labels indicating it as 'holotype' of 'M. marginatus'—an undescribed species so designated by F. M. Hull); RSA, Natal, 75 km WSW Estcourt, Cathedral Peaks, 1860m, 2♂♂, 7–31 December 1979, S. & J. Peck (sweep net), (ZMUC) (515979, 515988); RSA, KwaZulu-Natal, Vryheid Nature Reserve, 27o 45' S, 30o 46' E, 1300m, 1♀, 2 December 1999, T. Dikow (sweep net), (NMSA) (64705); RSA, Drakensberg Mountains, Cathedral Peak, blue pools, 28o 56' 45.14" S, 29o 12' 02.72" E, 7.12.2012, 2♂♂, 1♀, 7 December 2012, A. Vujić (sweep net), (FSUNS) (G2193, G2197, G2201). RSA, Gilboa Mountain, -29°17'16.67" S, 30°17'35.71" E, 6♂♂, 3♀♀, 22–23 December 2016, A. Vujić, S. Radenković, N. Veličković & T. Petanidou (sweep net), (CEUA, FSUNS & MZH) (ZA3_046, ZA3_047, ZA3_048, ZA3_051, ZA3_053, ZA3_054, ZA3_049, ZA3_050, ZA3_052)
Range (Fig 1). Species is distributed in southeastern part of RSA. Flight period: February and July to December.
Diagnosis. This species is very similar to Merodon drakonis sp. n. because of the short basoflagellomere (almost as long as wide) and tergite 2 with reddish-orange lateral spots, as well as conspicuous gray-green microtrichia on pleurae. The main differences in males are in the shapes of genitalia: posterior lobe of surstyle triangular, with apex pointed towards tip of hypandrium (Fig 3A: p); ventral margin of anterior lobe of surstyle almost straight (Fig 3: a); anterior lobe of surstyle with large inner thorn (Fig 3B: at). In females, the distance between the posterior ocellus and upper eye corner in M. melanocerus is larger than the distance between ocelli (Fig 9D); in M. drakonis sp. n., this distance is smaller (Fig 9G). Based on genetic data and characteristics of the male genitalia (Fig 10), the most closely related species to M. melanocerus is M. capensis (see diagnosis for M. capensis).
Description. Male. Head (Fig 9A, 9C and 9E): Antenna (Fig 9E) dark-brown, basoflagellomere 1.0–1.1 times as long as wide, 1.1 times longer than pedicel; arista about 2.2 times longer than basoflagellomere. Vertical triangle (Fig 9C) isosceles, 2.5 times longer than eye contiguity, predominately covered with long black pile, except pale yellow pile at anterior and posterior ends. Ocellar triangle (Fig 9C) equilateral. Eye contiguity about 10 ommatidia long. Eye pile as long as scape, mostly pale yellow, except for some black pile on apical 1/6.
Thorax: Scutum black with bronze lustre and indistinct whitish microtrichia anteriorly that turn into three longitudinal stripes (one narrow medially and two wide submedially ending as spots at the level of the transverse suture); scutellum black, but in some specimens with brown posterior rim. Microtrichia of pleurae conspicuous, the most developed on katepisternum, gray-green in colour. Halter brown. Legs dark brown-black, except for paler knees, and sometimes proximal 1/3 dorsally on tibiae. Metafemur (Fig 12C) slightly thickened and curved, with short and adpressed hairs in apical 1/3. Legs covered with pale yellow pile.
Abdomen: Tergites 2–4 black, with distinct white transverse band of microtrichia interrupted in the middle (in some specimens lateral sides of tergites and ground colour of transverse bands also orange-brown); tergite 2 with orange antero-lateral spots; pile on tergites mainly erect and yellow on lateral sides, but tergites 2–4 medially with black adpressed pile. Sternites brown, but in some specimens sternite 2 and anterior margin of sternite 3 can be paler.
Male genitalia (Fig 3): Ventral margin of anterior lobe of surstyle almost straight (Fig 3A: a); anterior lobe of surstyle with large inner thorn (Fig 3B: at); median part of surstyle with small inner thorn (Fig 3B: t); posterior lobe of surstyle triangular, with apex pointed towards tip of hypandrium (Fig 3A: p). Hypandrium relatively narrow, with smooth thecal ridge (Fig 3C: r).
Female. Similar to the male except for normal sexual dimorphism and for the following characteristics: frons without microtrichia except narrow line along eye margin and microtrichose area at antennal level; on vertex, distance between posterior ocellus and upper eye corner larger than distance between ocelli.
Key for species of the Merodon melanocerus subgroup.
Males
1. Eyes dichoptic (Fig 7C); basoflagellomere elongated, 1.5 times as long as wide (Fig 7D); male genitalia: posterior lobe of surstyle short, broad, triangular (Fig 13A: p)................................................................................. Merodon flavocerus Hurkmans sp. n.
- Eyes holoptic (as in Fig 9C); basoflagellomere short, 1.1 times as long as wide (as in Fig 9E); male genitalia: posterior lobe of surstyle with hook-like apex (as in Fig 3A: p).......................................................................................................................................... 2
2. Tergite 2 without large, distinct, orange lateral spots....................................................... 3
- Tergite 2 with large, distinct, orange lateral spots.............................................................. 4
3. Body hairs as usuall. Hypandrium with folded thecal ridge (Fig 2C: r), but without subapical lamellas and lateral wings.............................. Merodon capensis Hurkmans sp. n.
- Body hairs conspicuously thick, especially on vertical triangle and scutum. Hypandrium with smooth thecal ridge (Fig 8C), well-developed subapical lamellas (Fig 8C and 8D: s) and lateral wings (Fig 8C and 8D: w)................... Merodon commutabilis Radenković et Vujić sp. n.
4. Microtrichia on scutum well developed, including longitudinal stripe laterally on intraalar area till level of posterior end of postalar callus. Anterior lobe of surstyle with convex ventral margin (Fig 11A: a); hypandrium broad in apical fourth (Fig 11C), with oval subapical lamella (Fig 11C and 11D: s) and small lateral wings (Fig 11C and 11D: w)......................
........................................................................ Merodon drakonis Vujić et Radenković sp. n.
- Microtrichia on scutum indistinct, without microtrihose longitudinal stripe on intraalar area. Anterior surstyle lobe with almost straight ventral margin (Fig 3A: a) and large inner thorn (Fig 3B: at); hypandrium (Fig 3C) narrow in apical fourth, without subapical lamellas and lateral wings............................................... Merodon melanocerus Bezzi, 1915
Females
1. Tergite 2 black, at least laterally……......................................................................... 2
- Tergite 2 with large, distinct, orange lateral spots extending along lateral sides............... 3
2. Legs partly pale, at least pro- and mesotibiae at both ends, and basal two or three tarsomeres of pro- and mesotarsi; scutum with stripe of black pile between wing bases, the rest covered with pale yellow pile.................................. Merodon capensis Hurkmans sp. n.
- Legs black, sometimes metatarsi brown dorsally; scutum pilosity variable, can be covered with pale yellow or mixed black and pale yellow pile............................................................
................................................................ Merodon commutabilis Radenković et Vujić sp. n.
3. Basoflagellomere elongated, more than 1.5 times as long as wide (Fig 7E).......................
..................................................................................... Merodon flavocerus Hurkmans sp. n.
- Basoflagellomere short, 1.1 times as long as wide (as in Fig 9F)...................................... 4
4. Frons shiny, almost without microtrichia; distance between posterior ocelli and upper eye corner larger than distance between ocelli (Fig 9D)..........................................................
....................................................................................... Merodon melanocerus Bezzi, 1915
- Frons with lateral triangular microtrichose areas; distance between posterior ocelli and upper eye corner less than distance between ocelli (Fig 9G)....................................................
...................................................................... Merodon drakonis Vujić et Radenković sp. n.
Molecular evidence of species delimitation within the Merodon melanocerus subgroup
The molecular analysis included 23 specimens from the Merodon melanocerus subgroup. Amplification of both the 3’- and 5’- end of the COI gene was successful for all individuals. The dataset containing the COI 5’ region sequences had a final length of 639 bp. The final length of the dataset containing the COI 3’ region sequences was 657 bp. A combined dataset of the 3’ and 5’ COI regions was used for the phylogenetic analyses.
All obtained phylogenetic trees (ML, BI and MP) depict that the Merodon melanocerus subgroup belongs to the monophyletic Merodon desuturinus group (Figs 10 and 14 and S1 Fig). Inclusion of additional specimens from the genus Merodon in the phylogenetic trees allowed us to reveal four lineages (putative subgenera) within the Merodon genus; three previously established ones, albifrons+desuturinus, aureus (sensu lato) and avidus-nigritarsis, and one new lineage named natans (Fig 10). Within the M. desuturinus group, we observed clear differentiation of a Palaearctic lineage consisting of three species (M. desuturinus, M. cabarenensis and M. neolydicus) and two Afrotropical lineages consisting of the M. planifacies and the M. melanocerus subgroups, respectively. The positions of these three lineages within the M. desuturinus group differed on the MP tree compared to the ML and BI trees (these latter two exhibited the same topology). Based on the MP tree topology, the most divergent lineage within the group is the M. planifacies subgroup (S1 Fig). Unexpectedly, based on MP, the Palaearctic lineage was closer to the M. melanocerus subgroup that comprises specimens found exclusively in South Africa. In contrast, based on the ML and BI trees, the most divergent lineage was the Palaearctic one, and the M. planifacies and M. melanocerus subgroups were shown to be closely related.
The tree is drawn to scale, with branch lengths measured in the number of substitutions per site.
The molecular data confirmed delineation of four species within the M. melanocerus subgroup: M. melanocerus, M. capensis sp. n., M. drakonis sp. n. and M. commutabilis sp. n. None of the specimens showed shared haplotypes among different species. According to the phylogenetic trees, within the M. melanocerus lineage, M. drakonis sp. n. and M. commutabilis sp. n. are genetically closely-related species and form one cluster, whereas M. melanocerus and M. capensis sp. n. are more divergent and constitute another cluster. Genetic relatedness revealed by molecular analyses also reflected relationships defined according to the structure of male genitalia.
Discussion
The genus Merodon can be classified into about 20 monophyletic species groups, half of which were treated by Hurkmans [5] in his monograph. The majority of Merodon species are present in Eastern Europe and Asia Minor, which is associated with the high diversity of bulb species (their larval host plants) in these regions. Out of a total of ca. 160 species, less than 10 are known from the Afrotropical region. Only two species groups, aureus and desuturinus, have representatives in the Afrotropical region as well as in the Palaearctic.
We found that the desuturinus group consists of two clearly separate lineages based on both adult morphological and molecular data. The Palaearctic lineage includes four endemo-relicts: M. cabanerensis known only from a restricted highlands in central Spain and Morocco, M. desuturinus localized on high mountains in the Balkans, M. murorum from North-West Africa and M. neolydicus present in several countries in the Eastern Mediterranean (Greece, Turkey, Syria, Lebanon, Israel). Milankov et al. [26] observed low genetic variation in the species M. desuturinus and attributed this to the putative small sizes of the spatially isolated populations and its probable narrow ecological niche associated with possible historical events, especially genetic bottlenecks. This species occurs in Pleistocene refugia (high mountains of the Balkan Peninsula) and, together with the other three geographically-isolated Mediterranean taxa, can be considered oro-Mediterranean relicts.
The Afrotropical lineage comprises besides species M. cuthbertsoni, also two subgroups (M. melanocerus and M. planifacies). Contrary to all taxa of the desuturinus group, which have clear diagnostic adult morphological features, some members within the planifacies subgroup can only be delimited by genetic data. It is interesting that M. cuthbertsoni, a species from Zimbabwe (Southern Africa), also shares some distinct morphological characters with the South European endemo-relict, M. desuturinus. Unfortunately, there is no genetic data available for this South African endemic due to the fact that this species is only known from one specimen dated from 1925.
The Merodon melanocerus subgroup consists of five closely-related, though clearly morphologically and genetically separated, species distributed in the southeastern part of RSA. The most divergent species is M. flavocerus with several autapomorphic characters (basoflagellomere of antenna elongate and paler, katepisternum shiny, male eyes dichoptic, posterior lobe of surstyle shortened). Its distribution is restricted to the southern part of the Cape region. All records of this species date from the 20th century and, consequently, genetic data are missing. Additional species with an allopatric distribution is M. capensis sp. n., localized in the Western Cape Province. It is the most closely related to M. melanocerus species according to genetic data and features of male genitalia, but clearly different in external morphology. Structure of male genitalia represents the most conservative and stable morphological characters in hoverflies, important for species delimitation, but at the same time valuable for the revealing phylogenetic relationships. Resemblance in its shape reflects taxa relatedness [52, 53]. Another genetically close species, M. commutabilis sp. n. and M. drakonis sp. n., are also very similar in terms of structure of the male genitalia. During fieldwork, we observed that they appeared together, both sympatrically and synchronically, at some localities. Their distribution, and that of M. melanocerus, is associated with the southeastern part of RSA. Based on general external morphology, like the presence or absence of distinct orange lateral spots on tergite 2, less related species show greater resemblance to each other; for example M. drakonis sp. n. looks like M. melanocerus, or M. capensis sp. n. as M. commutabilis sp. n. However, there is no doubt that genetic data and structure of the male genitalia better reflect the true relationships than overall appearance, which often depends on habitat or other factors.
It is postulated that Merodon larvae all develop in the underground bulbs and rhizomes of geophytes (Amaryllidaceae, Iridaceae and Hyacinthaceae) or in the surrounding soil [5], based on data from eight species with described immature stages and three additional species whose larvae have been reared but are yet to be described (see review in [3, 4, 54]). Larvae of members of the Merodon desuturinus group are still undescribed but, according to field observations and unpublished data, they most probably also develop in plants of family Hyacinthaceae. Adults of M. desuturinus visit flowers of Ornithogalum and Scilla [26], and immature stages of the M. planifacies subgroup have been recorded in Merwilla bulbs. Family Hyacinthaceae comprises about 700–900 species, mainly distributed in Africa, Europe and SW Asia, with a single small genus in South America [55]. This distributional pattern suggests that diversification of this family began when North America was already clearly separated from Eurasia. Absence of the genus Merodon on the American continent can thus be linked to historical events in the Hyacinthaceae. However, the greatest diversity of Hyacinthaceae is found in South Africa [56], which is not in congruence with the exceptionally high diversity of Merodon in the Eastern Mediterranean. Migration of bulb species from southern Africa to northern Africa and Eurasia was possible during the late Early Miocene (c. 19 Mya.) when the Gomphotherium landbridge was formed and closed the Eastern Mediterranean seaway, giving rise to free exchange of flora and fauna between Africa and Eurasia [56, 57]. We suppose that diversification in the M. desuturinus group most probably happened much later, during fundamental shifts in African climate. Generally, the genus Merodon prefers warm, dry, open habitats with numerous bulb specimens. During the Pliocene-Pleistocene epoch, favorable conditions for Merodon species (increased aridity and open grasslands) in Africa [58] most probably allowed faunal transitions but, in the case of the Merodon desuturins group, this occurred in the opposite direction to the northward trajectory of the Hyacinthaceae; from the eastern Mediterranean (including SW Asia), one lineage migrated to South Africa and another to the western Palaearctic.
Separate position of the desuturinus group were revealed in earlier papers of Mengual et al. [13] and Milankov et al. [26], although each included only one species of the group (M. cabanerensis and M. desuturinus, respectively) in their analyses. Here, monophyly of the desuturinus group is confirmed (with an additional eight species analysed in relation to 27 other Merodon taxa), as well as its closest relationship to the albifrons group. Placement of the melanocerus subgroup on the obtained phylogenetic trees was variable. However, the MP tree showed a closer relationship of the M. melanocerus subgroup to species from the Palaearctic than to the M. planifacies subgroup which belongs to the Afrotropical lineage. In contrast, parametric ML and BI trees, commonly used to asses phylogeny, showed that the Afrotropical M. melanocerus and the M. planifacies subgoups are more closely related to each other than to Palaearctic species. To obtain a clearer picture of the relationships within the desuturinus group it will be necessary to obtain genetic data on M. flavocerus, M. cuthbertsoni and M. stevensoni, which is currently lacking since there are only old museum representatives available. We are planning future field investigations to fill missing gaps. Based on our phylogenetic analyses, we observe and name an additional lineage natans, in addition to three previously-established ones: albifrons+desuturinus, aureus (sensu lato) and avidus-nigritarsis described by Vujić et al. [9] and Šašić et al. [12]. These large lineages may reflect subgeneric divisions, but exploration of infrageneric structure and stability of these lineages will require a substantial expansion of taxa sampling for combined analyses of morphological and molecular data.
Supporting information
S1 Table. List of specimens used for molecular analysis and GenBank accession numbers for obtained sequences.
https://doi.org/10.1371/journal.pone.0200805.s001
(PDF)
S1 Fig. Strict consensus tree based on four equally parsimonious trees from analysis of combined 3'- and 5'- fragments of the COI gene.
Length 2040 steps, Consistency index (CI) 35, Retention index (RI) 66. Filled circles represent non-homoplasious changes and open circles are homoplasious changes.
https://doi.org/10.1371/journal.pone.0200805.s002
(GIF)
Acknowledgments
We are sincerely grateful to the curators that generously arranged for loans of both identified and unidentified material necessary to complete the present study. We are grateful to John O'Brien for English proofreading.
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