http://orcid.org/0000-0001-5568-8429
Figures
Abstract
Objective
The correlation of depleted blood through midline shift in acute subdural hematoma remains the most reliable clinical predictor to date. On the other hand, patient’s ABO blood type has a profound impact on coagulation and hemostasis. We conducted this study to evaluate the role of patient’s blood type in terms of incidence, clinical course and outcome after acute subdural hematoma bleeding.
Methods
100 patients with acute subdural hematoma treated between 2010 and 2015 at the author’s institution were included. Baseline characteristics and clinical findings including Glasgow coma scale, Glasgow outcome scale, hematoma volume, rebleeding, midline shift, postoperative seizures and the presence of anticoagulation were analyzed for their association with ABO blood type.
Results
Patient’s with blood type O were found to have a lower midline shift (p<0.01) and significantly less seizures (OR: 0.43; p<0.05) compared to non-O patients. Furthermore, patients with blood type A had the a significantly higher midline shift (p<0.05) and a significantly increased risk for postoperative seizures (OR: 4.01; p<0.001). There was no difference in ABO blood type distribution between acute subdural hematoma patients and the average population.
Conclusion
The ABO blood type has significant influence on acute subdural hematoma sequelae. Patient’s with blood type O benefit in their clinical course after acute subdural hematoma whereas blood type A patients are at highest risk for increased midline shift and postoperative seizures. Further studies elucidating the biological mechanisms of blood type depended hemostaseology and its role in acute subdural hematoma are required for the development of an appropriate intervention.
Citation: Dubinski D, Won S-Y, Behmanesh B, Brawanski N, Geisen C, Seifert V, et al. (2018) The clinical relevance of ABO blood type in 100 patients with acute subdural hematoma. PLoS ONE 13(10): e0204331. https://doi.org/10.1371/journal.pone.0204331
Editor: Santosh K. Patnaik, Roswell Park Cancer Institute, UNITED STATES
Received: March 9, 2018; Accepted: September 6, 2018; Published: October 4, 2018
Copyright: © 2018 Dubinski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: Data are from the The Clinical Relevance of ABO Blood Type in 100 Patients with Acute Subdural Hematoma study, whose authors may be contacted at daniel.dubinski@kgu.de.
Funding: Dr. Daniel Dubinski is a Else Kröner-Fresenius-Foundation scholar.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Isolated acute subdural hematoma (aSDH) is a form of intracranial bleeding defined by the presence of a blood collection between the dura and arachnoid layer, that requires immediate intensive treatment and the mortality rates are as high as 50–80%. [1] [2] Despite the presence of several outcome prediction models that are based on clinical parameters such as the initial Glasgow coma scale (GCS), present anticoagulation therapy, pupillary status and the time between onset and operation, the prediction of patient’s outcome remains challenging. [3] [4] [5] [6] Midline-shift on the other hand is an established parameter that is associated with a higher frequency of unfavourable outcome and death. [7] [8] Accordingly, additional risk factors are required for the prediction of short and long-term prognosis in these patients. In recent years several studies described the profound influence of the ABO blood type system on patients haemostasis and thrombosis. [9,10] The ABO blood type is determined by the glycosyltransferase on chromosome 9 that attaches monosaccharaides to the cell membrane on erythrocytes, platelets and vascular endothelial cells. The blood type O is a result of an inactive glycosyltransferase and is furthermore strongly associated with a reduction of the circulating procoagulatory vWF (von Willebrand factor) and F VIII (Factor VIII) plasma level of up to 30% compared to non-O blood type. [11] [12] As vWF binds and transports F VIII, this interaction is vital for platelet/matrix binding in terms of haemostatic response to vascular injury. A decreased plasma level of vWF and F VIII in patients with blood type O is therefore of direct clinical significance. [13][14] Coherent clinical studies confirmed the blood type O as a bleeding risk factor in several disciplines. [15][9] In line, patients with non-O blood type were shown to have an increased risk for suffering from VTE (venous thromboembolism), coronary heart disease and arterial thrombosis. [16,17] The influence of patients ABO blood type on clinical course and outcome after aSDH has not been investigated yet. We conducted this study to explore the role of patients ABO blood type as a new risk/prognostic factor.
Material and methods
Patients and data collection
The present study was approved by the clinical ethics committee of the University of Frankfurt. All patients over 18 years old with the diagnosis of isolated aSDH admitted to our University Hospital from 2010 to 2015 were identified retrospectively using the electronic database. All data were fully anonymized before access; hence no informed consent was required. Exclusion criterions included the lack of radiological data or ABO blood type, traumatic brain injury (TBI), pre-existing hematological disorders or hospital discharge in less than 24 hours after admission. Investigated medical record parameters included: ABO blood type, age at admission, gender, hematoma volume by ABC/2 formula, [18] midline shift, GCS at admission, GCS 24 hours after surgical treatment, rebleeding rate, GOS at 3 months, occurrence of postoperative seizures and anticoagulation treatment.
The radiological examinations were performed using the initial CT scan. For volume measurements the estimating formula ABC/2 was used. In patients with bilateral hematomas, both volumes were added together. For measurement of the midline shift and the width of the hematoma, the maximum diameter was used. In cases of clinically suspected epileptic seizures, we assessed the surface EEG recording that was reviewed by experienced neurologists who were blind to the patient’s outcome. Patient’s functional outcome was evaluated using the Glasgow Outcome Scale (GOS) at 3 months follow-up.
Study design
The present analysis is a retrospective, single centre observational study of isolated aSDH patients. The aim of the study was (1) to observe the incidence of the ABO blood type compared to the general German population, (2) examine the possible influence of ABO blood type on preoperative parameters and (3) to correlate the ABO blood type with patient’s outcome.
Statistics
Data analysis was performed with BiAS (Version 11.06.2017). For parametric parameters, the ANOVA test was used. For nonparametric parameters, the Wilcoxon-Mann-Whitney test was used. To assess the impact of the variables, odds ratio (OR) with 95% confidence intervals (CI) was calculated. Results with p ≤0.05 were considered statistically relevant.
Results
A total of 139 patients were eligible for this study. ABO blood type was not available in 39 cases. Thus, 100 patients (mean age 79 years, 33 female) were included in this study (study allocation displayed in Fig 1). Mean aSDH volume measured in the initial CT scan at admission was 105.16 ± 37.96 mm3 and a midline shift of 11.25 mm ± 5.14 mm. There was an even distribution between left and right hematoma side (47 vs. 49) and in 4 cases bilateral aSDH was diagnosed. Mean GCS at admission was 7.25 ± 3.87, postoperative 3.75 ± 5.02 and 7.75 ± 4.81 at discharge. 20% of the patients suffered from rebleeding and postoperative seizures were recorded in 44%. Mean GOS 3 months after discharge was 3 ± 1.61. 72% of the patients presented with anticoagulation at admission. For detailed patient’s baseline characteristics separated by ABO blood group see Table 1, S1 Table.
ABO blood type and the prevalence of aSDH
45 patients carry the blood type A (45%), followed by 43 patients with blood type O (43%), 6 patients with the AB blood type (6%) and 6 patients with blood type B (6%). There was no significant difference in blood type distribution between our analyzed cohort and ABO blood type distribution among the German population. The ABO distribution among German population and aSDH patients is displayed in Fig 2.
Advantage of blood type O in the clinical course of patients with aSDH
Postoperative seizures are strongly associated with poor outcome. In our cohort 14 (32%) patients with blood type O had epileptic seizures. This was the case for 28 (62%) of the patients with blood type A, one patient (17%) with blood type B, and one patient (17%) with the AB blood type. Therefore, patients with blood type O had a significantly decreased risk in the development of postoperative epileptic seizures (OR 0.43; 95% CI [0.09–0.98]; p = 0.035) (Table 2).
The preoperative midline shift was measured on the initial CT-scan as mentioned in the material section. Patients with blood type O had a mean midline shift of 6.5 ± 5.52 mm, blood type A patients had a mean midline shift of 11.7 ± 7 mm, blood type AB patients had a mean midline shift of 9.30 ± 4.77 mm whereas blood type B patients had 12.7 ± 2.37 mm. A significant lower midline shift was observed in blood type O patients (p = 0.007; median range 11.75–6.5 mm). (Table 1, Fig 3).
The left ordinate shows the mean midline shift of patients with aSDH and. The right ordinate shows the percentage of patients with postoperative seizures.
Blood type A is associated with an increased risk for aSDH sequelae
Among the analyzed parameters, the occurrence of postoperative seizures (verified via EEG as described in the methods section) was investigated. Statistical analysis showed a significant increase s in blood type A patients for the development of postoperative epileptic seizures (OR 4.01; 95% CI [1.73–9.27]; p = 0.0008).
Further, blood type A patients had a mean midline shift of 11.75 ± 7.02 mm in the preoperative CT scan. We found this parameter significantly increased compared to the other ABO blood types. (p = 0.029; median range 7.20–11.85 mm). (Table 1 Fig 3).
Patient’s present anticoagulation therapy does not influence aSDH volume, midline shift and outcome
To exclude the bias role of anticoagulation therapy in the comparison between the ABO blood types, we allocated the cohort according to present anticoagulation. Anticoagulation was present in significantly older patients (OR 6.18; 95% CI [11.55–22.45]; p = 0.0001) and was associated with poor postoperative outcome represented via GCS (OR 5.48; 95% CI [-8.17–3.82]; p = 0.0001) and at discharge (OR 5.48; 95% CI [3.82–8.17]; p = 0.0001) respectively. However, the presence of anticoagulation did not influence the mean subdural blood volume (OR 0.60; 95% CI [14.80–27.82]; p = 0.545), the mean midline shift (OR 1.88; 95% CI [0.12–5.33]; p = 0.561), the rebleeding rate (OR 1.21; 95% CI [0.39–3.71]; p = 0.789) nor the outcome analyzed through the GOS 3, month post-operative (OR 1; 95% CI [2.59–4.39]; p = 1). (See Table 3, Fig 3).
The influence of patients ABO blood type on clinical course and functional outcome
Patients with blood type O that were found to have a decreased midline shift and lower postoperative epileptic seizure incidence had interestingly no significant difference in the initial admission status represented through the GCS score, compared to non-O patients (p = 0.997; mean GCS 6). This effect remained to be not significant in the postoperative GCS evaluation (p = 0.727; mean GCS 3) and at discharge (p = 0.605; mean GCS 6; see Table 1). In terms of rebleeding rate no significant difference between O and non-O patients was found either (OR 0.65; 95% CI [0.23–1.82]; p = 0.291). Regarding the morbidity outcome expressed through the GOS at 3 months, no significant difference could be found between O and non-O patients either. (p = 0.504 mean GOS 3). Interestingly patients with AB blood type had a borderline significant better initial admission status expressed in a higher GCS score (p = 0.051 mean GCS 11.5) compared to non-AB patients. This effect was not detectable in the postoperative nor in the GCS status at discharge p = 0.269 and p = 0.666 respectively. Surprisingly GOS analysis at 3 months showed a significantly lower outcome in AB patients compared non-AB. (p = 0.018 mean range 3.00–0.50).
Discussion
Our analysis focused on the association between ABO blood type and the clinical course of aSDH patient’s. We identified several parameters that were significantly dependent on patient’s ABO blood type and describe our findings as additional new risk factors in aSDH burden.
aSDH is a serious condition that can cause mass effect, midline shift, and brain herniation. Prognostic factors are therefore highly needed in the clinical setting for predicting patient’s outcome and their clinical course. Several prognostic factors were described to be associated with poor outcome in aSDH including midline shift, initial poor GCS, seizures and antithrombotic medication. [4,8,19] On the other hand recent studies defined patients ABO blood type as a genetic bleeding risk factor. Clinical investigations and epidemiological data showed that patient’s with blood type O were at higher risk for clinically relevant hemorrhages, presumably due to the lowest amount of procoagulatory proteins in this blood group. [9,12,20] Vice versa non-O blood type patients were identified to be at an increased risk for developing VTE, coronary heart disease and arterial thrombosis. [17,21,22]
Interestingly, the Midline shift was significantly decreased in blood type O patients compared with non-O patients, presumably due to faster clot liquidation. Furthermore, the potential bias of anticoagulation therapy could be excluded in our analysis, which strengthen the clinical influence of ABO blood type in aSDH.
Counter-intuitively ABO blood type did not influence the postoperative rebleeding rate among our cohort. This finding could be in part explained by the rapid hemostaseologic interventions during patient’s intensive care treatment in terms of coagulation factor substitution and postoperative anticoagulation necessity.
Postoperative epileptic seizures are an important risk factor in aSDH contributing to patients’ morbidity and mortality. Whereas patients with blood type O had a significantly decreased risk for seizure development supposable through different clot properties and thereby lower cortex irritation, patients with blood type A had a significantly increased risk for postoperative seizures. So, especially patients with blood type A should receive a postoperative EEG.
In our cohort, ABO blood type did not significantly influence patient outcome evaluated through GCS score at admission, neither during intensive care treatment, nor at discharge. However, patients with the AB blood type (bearing the highest amount of procoagulatory proteins) showed the worst long-term outcome evaluated through the GOS scale at 3 months. Hemostaseological properties related to hematoma liquidation, patients’ recovery and rehabilitation progress could be responsible for this finding. Therefore, further analysis should prospectively identify embolic complications during follow-up period.
Today’s understanding of patients ABO blood type role in hemosatseology and thrombosis is gained mainly from extra-cranial cases. However, aSDH is a serious condition with high morbidity and mortality rates, especially among the elderly. [23,24] As the population ages, the prevalence of aSDH and patients with anticoagulation therapy is anticipated to identify the need for individualized hemostaseologic therapy, reliable risk and prognostic factors. [23,24]
To the best of our knowledge, data regarding the ABO blood type system and the clinical course after aSDH hemorrhage has not been investigated to date. Therefore, our data adds new insights in aSDH hemostaseology. However, our study has certain limitations. The present analysis was performed retrospective that is a subject for several bias itself. Further, several patients had to be excluded due to the lack of blood type data, making a selection bias possible. However, patient’s characteristics and a general blood group distribution indicate a good representation in our cohort. Furthermore, three patients were operated as an “outpatient procedure” (missing operative capacity in other hospitals with neurosurgical department or missing own ICU capacity in our hospital). In these patient’s also a lot of data is missing—especially history of anticoagulation, so these patients were excluded. Last to mention, data regarding long-term outcome further than 3 months after incidence was not included, excluding the possibility of the ABO blood type as a long-term outcome factor after aSDH.
Conclusion
To date several established risk factors for poor outcome after aSDH are available. However, as the morbidity and mortality rates after aSDH are high, precise outcome factors are urgently needed for treatment recommendation, especially among the elderly. Here, we present the patients ABO blood type as a genetic risk factor with direct clinical implication. Patients with blood type A are at a significantly high risk for increased midline shift and postoperative seizures. So, we recommend a postoperative EEG to identify seizure and to treat these patients adequately. The biological mechanisms of aSDH sequelae related to the ABO blood type need to be investigated further.
Supporting information
S1 Table. Patients characteristics.
Clinical parameters including ABO blood type.
https://doi.org/10.1371/journal.pone.0204331.s001
(XLSX)
References
- 1. Servadei F. Prognostic factors in severely head injured adult patients with acute subdural haematoma’s. Acta Neurochir (Wien). Springer-Verlag; 1997;139: 279–285.
- 2. Phuenpathom N, Choomuang M, Ratanalert S. Outcome and outcome prediction in acute subdural hematoma. Surg Neurol. 1993;40: 22–5. Available: http://www.ncbi.nlm.nih.gov/pubmed/8322172 pmid:8322172
- 3. Kotwica Z, Brzeziński J. Acute subdural haematoma in adults: an analysis of outcome in comatose patients. Acta Neurochir (Wien). 1993;121: 95–99.
- 4. Klun B, Fettich M. Factors influencing the outcome in acute subdural haematoma. A review of 330 cases. Acta Neurochir (Wien). 1984;71: 171–8. Available: http://www.ncbi.nlm.nih.gov/pubmed/6741634
- 5. Leitgeb J, Mauritz W, Brazinova A, Janciak I, Majdan M, Wilbacher I, et al. Outcome after severe brain trauma due to acute subdural hematoma. J Neurosurg. 2012;117: 324–333. pmid:22631691
- 6. Senft C, Schuster T, Forster M-T, Seifert V, Gerlach R. Management and outcome of patients with acute traumatic subdural hematomas and pre-injury oral anticoagulation therapy. Neurol Res. 2009;31: 1012–1018. pmid:19570326
- 7. Jacobs B, Beems T, van der Vliet TM, Diaz-Arrastia RR, Borm GF, Vos PE. Computed Tomography and Outcome in Moderate and Severe Traumatic Brain Injury: Hematoma Volume and Midline Shift Revisited. J Neurotrauma. 2011;28: 203–215. pmid:21294647
- 8. Bartels RH, Meijer FJ, van der Hoeven H, Edwards M, Prokop M. Midline shift in relation to thickness of traumatic acute subdural hematoma predicts mortality. BMC Neurol. 2015;15: 220. pmid:26496765
- 9. Dentali F, Sironi AP, Ageno W, Bonfanti C, Crestani S, Frattini F, et al. Relationship between ABO blood group and hemorrhage: a systematic literature review and meta-analysis. Semin Thromb Hemost. 2013;39: 72–82. pmid:23299820
- 10. Dentali F, Sironi AP, Ageno W, Crestani S, Franchini M. ABO blood group and vascular disease: an update. Semin Thromb Hemost. 2014;40: 49–59. pmid:24381150
- 11. Zhang H, Mooney CJ, Reilly MP. ABO Blood Groups and Cardiovascular Diseases. Int J Vasc Med. 2012;2012: 1–11. pmid:23133757
- 12. Zabaneh D, Gaunt TR, Kumari M, Drenos F, Shah S, Berry D, et al. Genetic Variants Associated with von Willebrand Factor Levels in Healthy Men and Women Identified Using the HumanCVD BeadChip. Ann Hum Genet. 2011;75: 456–467. pmid:21534939
- 13. TERRAUBE V, O’DONNELL JS, JENKINS P V. Factor VIII and von Willebrand factor interaction: biological, clinical and therapeutic importance. Haemophilia. 2010;16: 3–13. pmid:19473409
- 14. Lau CS, McLaren M, Belch JJ. Factor VIII von Willebrand factor antigen levels correlate with symptom severity in patients with Raynaud’s phenomenon. Br J Rheumatol. 1991;30: 433–6. Available: http://www.ncbi.nlm.nih.gov/pubmed/1747698 pmid:1747698
- 15. Kahr MK, Franke D, Brun R, Wisser J, Zimmermann R, Haslinger C. Blood group O: A novel risk factor for increased postpartum blood loss? Haemophilia. 2018; pmid:29877601
- 16. Dentali F, Sironi AP, Ageno W, Turato S, Bonfanti C, Frattini F, et al. Non-O blood type is the commonest genetic risk factor for VTE: results from a meta-analysis of the literature. Semin Thromb Hemost. 2012;38: 535–48. pmid:22740183
- 17. He M, Wolpin B, Rexrode K, Manson JE, Rimm E, Hu FB, et al. ABO blood group and risk of coronary heart disease in two prospective cohort studies. Arterioscler Thromb Vasc Biol. 2012;32: 2314–20. pmid:22895671
- 18. Won S-Y, Zagorcic A, Dubinski D, Quick-Weller J, Herrmann E, Seifert V, et al. Excellent accuracy of ABC/2 volume formula compared to computer-assisted volumetric analysis of subdural hematomas. PLoS One. 2018;13. pmid:29944717
- 19. Servadei F, Nasi MT, Giuliani G, Cremonini AM, Cenni P, Zappi D, et al. CT prognostic factors in acute subdural haematomas: the value of the “worst” CT scan. Br J Neurosurg. 2000;14: 110–6. Available: http://www.ncbi.nlm.nih.gov/pubmed/10889882 pmid:10889882
- 20. Wu O, Bayoumi N, Vickers MA, Clark P. ABO(H) blood groups and vascular disease: a systematic review and meta-analysis. J Thromb Haemost. 2008;6: 62–9. pmid:17973651
- 21. Streiff MB, Segal J, Grossman SA, Kickler TS, Weir EG. ABO blood group is a potent risk factor for venous thromboembolism in patients with malignant gliomas. Cancer. 2004;100: 1717–23. pmid:15073862
- 22. Streiff MB, Ye X, Kickler TS, Desideri S, Jani J, Fisher J, et al. A prospective multicenter study of venous thromboembolism in patients with newly-diagnosed high-grade glioma: hazard rate and risk factors. J Neurooncol. 2015;124: 299–305. pmid:26100546
- 23. Won S-Y, Dubinski D, Brawanski N, Strzelczyk A, Seifert V, Freiman TM, et al. Significant increase in acute subdural hematoma in octo- and nonagenarians: Surgical treatment, functional outcome, and predictors in this patient cohort. Neurosurg Focus. 2017;43. pmid:29088952
- 24. Won S-Y, Dubinski D, Bruder M, Cattani A, Seifert V, Konczalla J. Acute subdural hematoma in patients on oral anticoagulant therapy: management and outcome. Neurosurg Focus. 2017;43: E12. pmid:29088960