http://orcid.org/0000-0001-7687-9347
Correction
23 Jan 2019: The PLOS ONE Staff (2019) Correction: Comparative transcriptome analysis provides insights into dwarfism in cherry tomato (Solanum lycopersicum var. cerasiforme). PLOS ONE 14(1): e0211396. https://doi.org/10.1371/journal.pone.0211396 View correction
Figures
Abstract
Tomato, which can be eaten as a vegetable or fruit, is one of the most popular and nutritionally important crops around the world. Although most plants of the cherry tomato cultivar ‘Minichal’ have a normal phenotype, some plants have a stunted phenotype with reduced plant height, leaf size, and fruit size, as well as altered leaf and fruit shape. To investigate the molecular mechanisms underlying these differences, we generated RNA-seq libraries from pooled leaf samples of 10 normal (N) and 10 stunted (S) plants. Using the Illumina sequencing platform, we obtained a total of 115.45 million high-quality clean reads assembled into 35,216 genes and 35,216 transcripts. A total of 661 genes were differentially expressed between N and S plants. Of these, 420 differentially expressed genes (DEGs) were up-regulated, and 221 DEGs were down-regulated. The RNA-seq data were validated using quantitative reverse-transcription PCR. Enrichment analysis of DEGs using the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that the enriched pathways were involved in steroid biosynthesis, homologous recombination, and mismatch repair. Among these, three genes related to steroid biosynthesis, including 3BETAHSD/D2, DIM and DWF5 were down-regulated in S compared to N. Of these, DIM and DWF5 are known to be involved in brassinosteroid biosynthesis. Our results thus provide a useful insight into dwarfism in cherry tomato, and offer a platform for evaluating related species.
Citation: Rahim MA, Jung H-J, Afrin KS, Lee J-H, Nou I-S (2018) Comparative transcriptome analysis provides insights into dwarfism in cherry tomato (Solanum lycopersicum var. cerasiforme). PLoS ONE 13(12): e0208770. https://doi.org/10.1371/journal.pone.0208770
Editor: Ji-Hong Liu, Key Laboratory of Horticultural Plant Biology (MOE), CHINA
Received: September 13, 2018; Accepted: November 21, 2018; Published: December 7, 2018
Copyright: © 2018 Rahim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The data set supporting the results of this article is available in the the Sequence Read Archive (SRA) repository of NCBI under the accession number SRP152091.
Funding: This study was funded by the by the Golden Seed Project (Center for Horticultural Seed Development, grant no. 213007-05-2-CG100) of the Ministry of Agriculture, Food and Rural affairs in the Republic of Korea (MAFRA) (Recipient: Ill-Sup Nou). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Cultivated tomato (Solanum lycopersicum L.) is nutritionally rich, economically important, and widely grown around the world. It is ranked as the second most-consumed vegetable after the potato [1]. Tomato can be consumed fresh or in processed food items such as ketchup, paste, juice, pizza sauce, and soup. The ripe tomato fruit is abundant in lycopene, a red carotenoid pigment that has antioxidant properties, which help to protect against heart diseases, and lung and prostate cancer [2–5]. It also contains other carotenoids, including beta-carotene, neurosporene, lutein, and zeaxanthin, which support the human immune system [6]. Tomato is also a good source of vitamins, minerals and bioactive phenolic compounds, including vitamin C, vitamin K, tocopherols, folate, and potassium [7].
Cherry tomato (Solanum lycopersicum var. cerasiforme) is an ancestor of the domesticated form of cultivated tomato [8]. The content of bioactive compounds is generally higher in cherry-type tomatoes than in large ones [9], and fresh cherry tomatoes contain higher levels of nutrient and phenolic compounds than their processed products [9]. Therefore, the rate of consumption of fresh tomato fruit is increasing rapidly, and cherry tomatoes, in particular, are becoming increasingly popular as a fresh salad food because of their high nutritional quality.
In this study, we characterized a stunted phenotype of the cherry tomato cultivar ‘Minichal’, which exhibits defective growth and development, including reduced internode length, a highly branched inflorescence and reduced fruit size compared to the normal ‘Minichal’ phenotype. This dwarfism ultimately reduces the economic value of this tomato cultivar.
Several previous reports have demonstrated that mutations in hormone biosynthesis and signaling genes can result in dwarfism in plants. For example, Koornneef and van der Veen [10] characterized GA5 (GA20ox1) mutants in Arabidopsis. GA5 is involved in gibberellic acid (GA) biosynthesis, and its mutation leads to plant dwarfism. Timpte et al. [11] described a mutation in axr2, which affects an auxin responsive protein and causes dwarfism in Arabidopsis characterized by reduced cell length and number in both hypocotyls and inflorescences, and also by reduced epidermal cell size. Notably, exogenous treatment with auxin was able to rescue the mutant phenotype [12].
In addition, several brassinolide (BL) steroids, which are collectively known as brassinosteroids (BRs) [13,14] are crucial for normal growth and development in plants [15]. BL is the most active form of BR, and is the end product of the BR biosynthesis pathway [13,16]. BRs are involved in a variety of physiological processes, including promotion of cell elongation, cell differentiation, retardation of senescence, promotion of ethylene biosynthesis, modulation of stress responses, and regulation of gene expression [17]. They are biosynthesized through two alternate pathways; the early and late C-6 oxidation pathways [15,17,18], have been studied in plant species including Arabidopsis, pea, rice, and tomato [18]. The enzymes catalyzing the BR biosynthesis pathway have been particularly well characterized in Arabidopsis, as have BR biosynthesis mutants that result in a dwarf phenotype, including det2 [19], dwf1 [20,21], cpd [22], dwf4 [23,24], dwf5 [25], dwf7 [26], and sax1 [27], and BR signaling and perception mutants [18]. Two BR biosynthesis dwarf mutants, BR‐deficient dwarf1 (brd1) and ebisu dwarf (d2), which exhibit stem and leaf elongation abnormalities, have been reported in rice [28]. In addition, a dwarf mutant with reduced BR levels, lk, has been reported in pea, and exogenous application of brassinolide restores it to a normal growth phenotype [18]. In cultivated tomato, two dwarf mutants, dumpy (dpy) and dwarf (d), have been reported, both of which are defective in BR biosynthesis [12].
Until now, little was known about the molecular mechanisms underlying dwarfism in cherry tomato. To gain insight into these molecular mechanisms, we used the Illumina sequencing platform to carry out transcriptomic analysis of leaves from normal (N) and stunted (S) tomato plants of the cultivar ‘Minichal’. We identified differentially expressed genes that might be involved in dwarfism of this cherry tomato cultivar ‘Minichal’. We further validated their expression pattern by qRT-PCR. These results provide a basis for identifying the key genes involved in tomato dwarfism.
Materials and methods
Plant materials
Two different phenotypes of the tomato cultivar ‘Minichal’, which included normal (N) plants with regular growth and development, and stunted (S) plants with reduced plant growth and development, were used (Fig 1). These lines were grown in a glasshouse at the Department of Horticulture, Sunchon National University, Suncheon, Republic of Korea. Young leaves were sampled from 10 individual plants for each phenotypic category (N and S). Leaves were pooled and frozen in liquid nitrogen before storing them at –80°C until required.
A) Leaves; B) inflorescences; C) fruits; D) mature plants.
RNA extraction and library construction for transcriptome analysis
Total RNA was isolated from 100 mg finely powdered leaf tissues using the RNeasy Mini Kit (Qiagen, USA). The quantity and integrity were checked with a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, Delaware, USA) and an Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA). Two RNA-seq libraries were constructed by Theragen Bio Institute (Suwon, South Korea) using the TruSeq RNA Library Prep Kit (Illumina Inc.) and RNA samples with a RIN (RNA integrity number) greater than 7. RNA sequencing was performed using an Illumina HiSeq 2000 platform (Illumina Inc.). RNA sequencing data were analyzed according to the method described by Trapnell et al. [29].
Quantification of expression patterns and differentially expressed genes
Reference genome and gene model annotation files for tomato (Solanum lycopersicum) were retrieved from the Ensembl database (https://plants.ensembl.org/). Clean reads were mapped to the reference genome using TopHat (v.2.1.1; http://ccb.jhu.edu/). Assembled genes were searched against the Swiss-Prot database and Gene Ontology (GO) categories. Gene expression patterns and differential expression were determined using Cufflinks (v.2.0.1; http://cufflinks.cbcb.umd.edu/), as previously reported by Trapnell et al. [29]. The expression level was normalized by the number of fragments per kilobase of exon per million mapped reads (FPKM). Differentially expressed genes (DEGs) were detected using DEGseq [30] with an adjusted p < 0.005 and q < 0.05. All DEGs were subjected to GO analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis using WebGestalt [31] and DAVID (https://david.ncifcrf.gov/).
Validation of RNA-seq data by qRT-PCR
The expression patterns of eight genes were selected for further validation by quantitative reverse transcription PCR (qRT-PCR). cDNA was synthesized from 2 μg of high-quality total RNA using SuperScript III (Invitrogen, Gaithersburg, MD). The qRT-PCR reaction was carried out using 50 ng cDNA and a gene-specific primer (S1 Table) with 2x SyGreen Mix Lo-ROX (qPCRBIO; PCR Biosystems, London, UK) and a LightCycler 96 instrument (Roche, Mannheim, Germany). The reaction conditions were: 95°C for 5 min, then 50 cycles at 95°C for 10 s, 60°C for 10 s, and 72°C for 15 s. Cq values obtained from qRT-PCR were analyzed with LightCycler 96 software (Roche, Germany). The mean normalized expression was determined by the comparative 2−ΔΔCt method [32], where Elongation factor-1alpha (EF-1alpha) was used as an internal control gene for Solanum lycopersicum.
Results
Overview of RNA sequencing
Leaves at similar stages of growth were collected and pooled from normal (N) and stunted (S) tomato plants for RNA isolation. Their transcriptomes were profiled using the Illumina sequencing platform. We obtained 117.995 million paired-end raw reads (Table 1). Subsequently, adapters, low-quality reads, and ambiguous reads were removed (Fig 2A). A final total of 115.450 million high-quality clean reads were obtained (54.755 and 60.695 million for N and S, respectively) (Table 1).
A) Classification of raw reads; B) distribution of genes’ coverage for normal pool (N); C) distribution of genes’ coverage for stunted pool (S).
A total of 97.6% reads from N plants, and 98.0% reads from S plants, were mapped to the S. lycopersicum reference genome (Ensembl). On average, 91.6% and 91.5% reads, respectively, uniquely mapped to the reference database. High-quality clean reads were assembled into 35,216 transcripts and 35,216 genes (Table 1). Among the annotated genes, 72% and 71% had 90–100% coverage in N and S tomato libraries, respectively (Fig 2B and 2C), indicating that the distributions of reads were similar between tomato libraries. Both the samples had Q20 scores (indicating Phred-like quality) greater than 96%, indicating the high quality of the RNA sequencing. These high quality transcriptomic data from N and S plants therefore provide a basis for identifying the key genes involved in tomato dwarfism.
Differentially expressed genes between normal and stunted tomato pools
A total of 661 differentially expressed genes (DEGs) between N and S tomato plants were identified using the R package DEGseq (S2 Table) [30]. Of these DEGs, 420 genes were up-regulated, and 241 genes were down-regulated in S versus N (Fig 3). However, 32 DEGs were found to be expressed in S only, and 108 in N only (S2 Table). The distribution of up-regulated and down-regulated genes is shown using a volcano plot (Fig 4).
X-axis and y-axis represent log2 fold-change differences between the compared samples and statistical significance as the negative log of DEG P-values, respectively. The significantly up-regulated and down-regulated genes are indicated with red and blue dots, respectively, while non-significant genes are shown as black dots.
Functional classification of DEGs
Using GO analysis, DEGs were classified into three main categories: biological processes, cellular components, and molecular functions; and 50 functional groups (Fig 5). In the biological process category, ‘metabolic process’ (GO:0008152), ‘response to stimulus’ (GO:0050896), and ‘biological regulation’ (GO:0065007) were the most important GO terms identified; in the cellular components category, ‘membrane’ (GO:0016020) and ‘nucleus’ (GO:0005634) were most important; and in the molecular function category, ‘protein binding’ (GO:0005515), ‘ion binding’ (GO:0043167), ‘nucleic acid binding’ (GO:0003676), and ‘hydrolase activity’ (GO:0016787) were most frequently identified.
To obtain insight into the biological significance of identified DEGs, GO enrichment analysis was performed using the Gene Ontology database (http://www.geneontology.org/). Enriched GO terms for genes that were up-regulated and down-regulated between N and S tomato plants are shown in Fig 6. GO enrichment analysis revealed that ‘catalytic activity’ and ‘metabolic process’ were the most often enriched GO terms for both up-regulated and down-regulated genes (S3 Table).
GO enrichment of up-regulated (A) and down-regulated (B) genes in leaves of stunted (S) and normal (N) cherry tomato plants (cv. ‘Minichal’). The P-value was corrected as P < 0.5.
Identified DEGs were also subjected to functional annotation clustering at the highest level of stringency using the DAVID database (https://david.ncifcrf.gov/). The analysis showed 22 clusters (S4 Table) with an enriched score ranging from 0.02 to 2.37. Of these, six clusters had an enrichment score greater than 1.0 (Table 2). The most enriched terms were ‘steroid biosynthesis’, ‘WRKY transcription factor’ (TF), ‘DNA damage/repair’, ‘tetratricopeptide repeat’ (TPR), ‘MADS-box TF’, and ‘mitogen-activated protein kinases’ (MAPK).
DEG pathway analysis was done using DAVID and the KEGG pathway database, using default threshold parameters except for EASY, which was set at >0.3. The results indicated that ‘steroid biosynthesis’, ‘homologous recombination’, ‘mismatch repair’, ‘DNA replication’, ‘protein export’, ‘glucosinolate biosynthesis’, ‘vitamin B6 metabolism’, ‘nucleotide excision repair’, ‘2-oxocarboxylic acid metabolism’, ‘zeatin biosynthesis’, and ‘cutin, suberin, and wax biosynthesis’ were highly enriched pathways (Table 3), with the most significantly enriched pathways being ‘homologous recombination’ and ‘steroid biosynthesis’.
Expression pattern of genes related to homologous recombination, steroid and cytokinin/zeatin biosynthesis
Transcript levels of four genes, RPA3B (Solyc09g009900.2), RPA2B (Solyc10g081830.1), XRCC3 (Solyc07g055170.1) and RPA1E (Solyc03g013260.1)—all related to homologous recombination—were up-regulated in stunted (S) ‘Minichal’ cherry tomatoes compared to those with a normal (N) phenotype. On the contrary, three genes related to the steroid biosynthetic pathway, 3BETAHSD/D2 (Solyc02g081730.2), DWF5 (Solyc06g074090.2), and DIM (Solyc02g069490.2), were down-regulated in S compared to N (Fig 7). The gene adenylate isopentenyl transferase 3 (IPT3), which is involved in cytokinin biosynthesis, and cytokinin oxidase 3 (CKX3), which catalyzes the degradation of cytokinins, were up-regulated in S compared to N (Fig 7), although expression of CKX3 was higher than that of the IPT3.
FPKM values were obtained from RNA-seq data. Red and blue colors represent the maximum and the minimum values, respectively.
Expression patterns of other hormone-related genes
Among the DEGs, four genes related to the auxin signaling pathway, IAA14 (Solyc09g083290.2), AX6B_SOYBN (Solyc04g053010.1), AXX15_SOYBN (Solyc11g011650.1), and 12KD_FRAAN (Solyc02g077880.2), were up-regulated, and two genes, AIR12 (Solyc09g056390.1), and AXX15_SOYBN (Solyc04g053000.1), were down-regulated in S compared to N (Fig 8). Two ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), and 1-aminocyclopropane-1-carboxylate oxidase 3 (ACO3), were highly expressed in S compared to N (Fig 8). Furthermore, ethylene responsive factor (ERF) genes that lie downstream of the ethylene signaling pathway were also differentially expressed. Among these downstream genes, ERF13 (Solyc01g090340.2) was the most highly expressed and was down-regulated in S compared to N. Expression of the remaining three ERFs was very low, and two of these (ERF003, Solyc03g117130.2, and ERF13, Solyc01g090310.2) were not expressed in ‘N at all.
FPKM values were obtained from RNA-seq data. Red and blue colors represent the maximum and minimum values, respectively.
Expression patterns of WRKY TF, MADS-box TF, MAPK and TPR-related genes
The expression of four WRKY TF genes, WRKY 40 (Solyc03g116890.2), WRKY 41 (Solyc01g095630.2), WRKY 50 (Solyc08g062490.2), and WRKY 51 (Solyc12g056750.1), were up-regulated in S compared to N (Fig 9). Among the MADS-box TF genes, AGL36 (Solyc01g103550.1), and SEPALLATA 2 (Solyc02g089200.2) were up-regulated, while SVP (Solyc04g076280.2), and AGL19 (Solyc08g080100.2), were down-regulated in S compared to N (Fig 9). The YDA (Solyc06g036080.2) gene, which encodes a mitogen-activated protein kinase (MAPK), was down-regulated in S (Fig 9). Transcript levels of three tetratricopeptide repeat (TPR)-like genes, FKBP65 (Solyc10g078250.1), LPA1 (Solyc09g063140.2), and NOXY38 (Solyc05g050630.2), were down-regulated, while ATSDI1 (Solyc06g007970.2) was up-regulated in S compared to N (Fig 9).
FPKM values were obtained from RNA-seq data. Red and blue colors represent the maximum and minimum values, respectively.
Validation of RNA-seq data
To test the reliability of RNA-seq results, qRT-PCR was used to measure the expression of eight genes with the same RNA samples used for RNA-seq. Relative expression of the tested genes was consistent with the RNA-seq data (Fig 10), confirming the efficiency and accuracy of the RNA-seq experiments.
Error bar indicates ±SE of the means of three replicates.
Discussion
Deep sequencing-based RNA-seq technology has made it possible to rapidly analyze large genomic datasets and quantify transcriptomes [33]. This high-throughput, next-generation sequencing technology has become a powerful tool for analyzing transcriptomes, and has been successfully used for both human and plant transcriptomes [34]. Global gene expression patterns can be determined using RNA-seq in samples of tissues at different developmental stages, with contrasting characteristics, or in response to different environmental stimuli [33,35,36]. In this study, we observed a stunted phenotype of the cherry tomato cv. ‘Minichal’, which is characterized by alterations in plant height, leaf size/shape, and fruit size/shape compared to the normal phenotype. We used RNA-seq to profile the transcriptomes of normal (N) and stunted (S) cherry tomatoes of this cultivar. We obtained almost 115.450 million high-quality clean reads, which were assembled into 35,216 transcripts (Table 1).
Our results identified 661 DEGs between the pooled RNA of N and S tomato plants (S2 Table). Subsequently, GO enrichment revealed that ‘metabolic process’ and ‘catalytic activity’ were the most enriched GO terms for both up-regulated and down-regulated genes between N and S (S3 Table).
To obtain further insight into the biological functions of these DEGs, GO functional annotation and KEGG pathway enrichment analysis were performed using the DAVID tool. Functional annotation clustering of DEGs revealed that the most enriched GO terms were associated with the sterol biosynthesis process (GO:0016126; enrichment score 2.37) (Table 2). KEGG pathway enrichment also indicated that ‘steroid biosynthesis’ and ‘homologous recombination’ were the most enriched pathways (Table 3). These results clearly suggest that genes related to steroid biosynthesis might be involved in dwarfism in S tomatoes.
Several studies have been conducted on plant dwarfism. Dwarfism is sometimes advantageous; for example in cereal crops—specifically rice and wheat, where lodging decreases crop productivity [37]. However, in tomato, dwarfism is deleterious because it reduces both quality and productivity. Plant dwarfism results from many genetic defects, mostly associated with hormone biosynthesis and perception [12]. Functional analysis of several genes related to dwarfism has previously been reported, including genes related to BR and GA biosynthesis and perception in different plant species [22,28,38–41].
BRs play significant roles in plant growth and development, and are biosynthesized via multiple parallel pathways starting with the precursor campesterol [15,24,42,43]. Defects in the BR biosynthesis/signaling cause dwarfism in plants [13]. For example, in Arabidopsis, dwarf5 (dwf5) mutants have a mutation in the gene for the enzyme 7-dehydrocholesterol reductase, which disrupts the sterol Δ7 reduction step and leads to dwarfism [25]. Likewise in Arabidopsis, dwarfism occurs in dwf4 mutants, which have a mutation in the gene encoding steroid 22α hydroxylase (CYP90B1), which is involved in 22α‐hydroxylation of the BR pathway [24].
In this study, three DEGs, 3beta-hydroxysteroid-dehydrogenase (3BETAHSD/D2, Solyc02g081730.2), 7-dehydrocholesterol reductase (DWF5, Solyc06g074090.2), and delta(24)-sterol reductase (DIM, Solyc02g069490.2)—all related to the steroid hormone biosynthesis pathway—were down-regulated in S compared to N plants (Figs 7 and 10). We also checked the expression patterns of these three genes in leaf, inflorescence, and fruit tissues of the cherry tomato cv. ‘Minichal’ (Fig 11). The result indicated that the expression of these steroid biosynthesis genes was higher in N than S plants. In N, expression was highest in leaf and lowest in fruit, while in S, expression was similar in leaves and inflorescences, but was drastically reduced in fruits.
Error bar indicates ±SE of the means of three replicates.
The protein interaction network of three steroid biosynthesis-related genes, 3beta-hydroxysteroid-dehydrogenase (3BETAHSD/D2), 7-dehydrocholesterol reductase (DWF5), and delta(24)-sterol reductase (DIM), highlighted their possible contribution to dwarfism of tomato plants (Fig 12). In S cherry tomatoes, these genes might be involved in dwarfism by directly or indirectly affecting steroid biosynthesis. In apple plants (Malus × domestica), colchicine-induced autotetraploid plants showed dwarfism, with decreased levels of indole-3-acetic acid (IAA) and BR compared to diploid plants. Furthermore, digital gene expression analysis of these apple plants revealed that DEGs between them were mostly related to IAA and BR biosynthesis pathways [44]. In Arabidopsis, a biosynthetic defect in dwf1, which encodes delta(24)-sterol reductase, resulted in dwarfism with reduced levels of BR synthesis compared to the wild type [45]. A similar dwf1 dwarf mutant has been reported in pea [46]. The dwf5 mutant, which is defective in BR biosynthesis, also showed a dwarf phenotype in Arabidopsis [25]. In rice, the dwarf mutant ebisu dwarf (d2) is deficient in BR biosynthesis and caused dwarfism, but exogenous application of BL restored the normal phenotype [39]. In tomato, the BR biosynthesis-defective mutant Dwarf (D), which harbors a mutation in cytochrome P450 (P450), exhibits dwarfism, while complementation 35S::D lines restore the normal phenotype [12,40]. Similar dwarf mutant dumpy (dpy) resulted from the mutation of mutation in the C-23 steroid hydroxylase (cpd) gene has also been reported in tomato by Kaka et al. [47]. However, our reported genes (3BETAHSD/D2, DWF5 and DIM) for dwarfism of cherry tomato are different from those previously reported mutants like D and dpy. Up-regulation of cytokinin dehydrogenase 3 (CKX3) in S tomatoes led to a higher rate of cytokinin degradation in these plants. Reid et al. [48] reported that the cytokinin content was negatively regulated by the activity of CKX3 in the root of Lotus japonicus ckx3 mutants.
The auxin signaling genes AIR12 (Solyc09g056390.1), and AXX15 (Solyc04g053000.1), but not IAA14, were down-regulated in N (Fig 8). This suggests that auxin signaling genes might be affected, leading to defective plant development. A similar result has been reported in tetraploid apple [44].
Previous studies have revealed that GA has an effect on plant growth and development. For example, exogenous treatment of dwarf pea and dwarf maize seedlings with GA3 enhanced longitudinal growth rates [49]. However, we found no DEGs related to GA biosynthesis in this study.
The up-regulation of two 1-aminocyclopropane-1-carboxylic acid oxidase genes, ACO1 and ACO3, which are involved in the final step of ethylene biosynthesis, suggests higher levels of ethylene production in S tomatoes, which might affect plant growth and development. Ethylene overproduction has been shown to inhibit plant growth in Arabidopsis [50,51].
The ‘short vegetative phase’ (SVP) group of MADS-box genes, such as OsMADS22, OsMADS47, and OsMADS55, have been shown to act as negative regulators of BR responses in rice [52]. The double and triple RNAi plants (OsMADS22–OsMADS55 and OsMADS22–OsMADS47–OsMADS55, respectively) showed reduced stem elongation. Unexpectedly, in this study, we also found that the expression of the MADS-box genes SVP (Solyc04g076280.2) and AGL19 (Solyc08g080100.2) was down-regulated, and AGL36 (Solyc01g103550.1) and SEPALLATA2 (Solyc02g089200.2) were up-regulated in S compared to N plants (Fig 9). Overexpression of OsMADS1 causes dwarfism in rice via irregular activation of BR and GA synthesis pathways [53].
Kim et al. [54] demonstrated that BR controls stomatal development by activating mitogen-activated protein kinase kinase kinase (MAPKKK) in Arabidopsis. Likewise, we found that MAPKK was up-regulated in S compared to N tomatoes (Fig 9).
Conclusions
We conducted comparative transcriptome analysis using normal and stunted plants of the cherry tomato cv. ‘Minichal’. DEGs related to steroid biosynthesis may be involved in dwarfism in this tomato cultivar. To best of our knowledge, this is the first comparative transcriptome analysis for plant dwarfism in tomato. Our results provide insight into the molecular mechanism of dwarfism and lay the foundation for future studies in related species.
Supporting information
S1 Table. Primers used for qRT-PCR validation.
https://doi.org/10.1371/journal.pone.0208770.s001
(XLSX)
S2 Table. Differentially expressed genes between normal (N) and stunted (S) tomato plants.
https://doi.org/10.1371/journal.pone.0208770.s002
(XLSX)
S3 Table. GO terms overrepresented in up-regulated and down-regulated genes, and number of genes belonging to each term.
https://doi.org/10.1371/journal.pone.0208770.s003
(XLSX)
S4 Table. Twenty-two functional annotation clusters of DEGs.
https://doi.org/10.1371/journal.pone.0208770.s004
(XLSX)
Acknowledgments
This research was financially supported by the Golden Seed Project (Center for Horticultural Seed Development, grant no. 213007-05-3-CG100) of the Ministry of Agriculture, Food and Rural Affairs in the Republic of Korea (MAFRA).
References
- 1. Foolad MR. Genome mapping and molecular breeding of tomato. Int J Plant Genomics. 2007;2007: 64358. pmid:18364989
- 2. Müller L, Caris-Veyrat C, Lowe G, Böhm V. Lycopene and its antioxidant role in the prevention of cardiovascular diseases—a critical review. Crit Rev Food Sci Nutr. 2016;56:1868–1879. pmid:25675359
- 3. Burton-Freeman BM, Sesso HD. Whole food versus supplement: comparing the clinical evidence of tomato intake and lycopene supplementation on cardiovascular risk factors. Adv Nutr An Int Rev J. 2014;5:457–485. pmid:25469376
- 4. Holick CN, Michaud DS, Stolzenberg-Solomon R, Mayne ST, Pietinen P, Taylor PR, et al. Dietary carotenoids, serum beta-carotene, and retinol and risk of lung cancer in the alpha-tocopherol, beta-carotene cohort study. Am J Epidemiol. 2002;156:536–47. pmid:12226001
- 5. Holzapfel NP, Holzapfel BM, Champ S, Feldthusen J, Clements J, Hutmacher DW. The potential role of lycopene for the prevention and therapy of prostate cancer: From molecular mechanisms to clinical evidence. Int J Mol Sci. 2013; 4(7):14620–14646.
- 6. Erge HS, Karadeniz F. Bioactive compounds and antioxidant activity of tomato cultivars. Int J Food Prop. 2011;14:968–977.
- 7. Ilahy R, Hdider C, Lenucci MS, Tlili I, Dalessandro G. Phytochemical composition and antioxidant activity of high-lycopene tomato (Solanum lycopersicum L.) cultivars grown in Southern Italy. Sci Hortic. 2011;127:255–261.
- 8. Ranc N, Muños S, Santoni S, Causse M. A clarified position for Solanum lycopersicum var. cerasiforme in the evolutionary history of tomatoes (Solanaceae). BMC Plant Biol. 2008;8:130. pmid:19099601
- 9. Choi SH, Kim HR, Kim HJ, Lee IS, Kozukue N, Levin CE, et al. Free amino acid and phenolic contents and antioxidative and cancer cell-inhibiting activities of extracts of 11 greenhouse-grown tomato varieties and 13 tomato-based foods. J Agric Food Chem. 2011;59:12801–12814. pmid:22070764
- 10. Koornneef M, van der Veen JH. Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh. Theor Appl Genet. 1980;58:257–263. pmid:24301503
- 11. Timpte CS, Wilson AK, Estelle M. Effects of the axr2 mutation of Arabidopsis on cell shape in hypocotyl and inflorescence. Planta. 1992;188:271–278. pmid:24178265
- 12. Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, et al. The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci U S A. 1999;96:1761–1766. pmid:9990098
- 13. Chung Y, Choe S. The regulation of brassinosteroid biosynthesis in Arabidopsis. Crit Rev Plant Sci. 2013;32:396–410.
- 14. Fujioka S, Sakurai A. Brassinosteroids. Nat Prod Rep. 1997;14:1–10. Available: http://pubs.rsc.org/en/Content/ArticlePDF/1997/NP/NP9971400001 pmid:9121728
- 15. Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, et al. Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis. Plant Physiol. 2001;126:770–779. pmid:11402205
- 16. Grove MD, Spencer GF, Rohwedder WK, Mandava N, Worley JF, Warthen JD, et al. Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature. 1979;281:216–217.
- 17. Clouse SD, Sasse JM. BRASSINOSTEROIDS: Essential regulators of plant growth and development. Annu Rev Plant Physiol Plant Mol Biol. 1998;49: 427–451. pmid:15012241
- 18. Nomura T, Jager CE, Kitasaka Y, Takeuchi K, Fukami M, Yoneyama K, et al. Brassinosteroid deficiency due to truncated steroid 5alpha-reductase causes dwarfism in the lk mutant of pea. Plant Physiol. 2004;135:2220–9. pmid:15286289
- 19. Li J, Nagpal P, Vitart V, McMorris TC, Chory J. A role for brassinosteroids in light-dependent development of Arabidopsis. Science. 1996;272:398–401. pmid:8602526
- 20. Klahre U, Noguchi T, Fujioka S, Takatsuto S, Yokota T, Nomura T, et al. The Arabidopsis DIMINUTO/DWARF1 gene encodes a protein involved in steroid synthesis. Plant Cell. 1998;10:1677–90. Available: http://www.ncbi.nlm.nih.gov/pubmed/9761794 pmid:9761794
- 21. Kauschmann A, Jessop A, Koncz C, Szekeres M, Willmitzer L, Altmann T. Genetic evidence for an essential role of brassinosteroids in plant development. Plant J. 1996;9:701–713.
- 22. Szekeres M, Németh K, Koncz-Kálmán Z, Mathur J, Kauschmann A, Altmann T, et al. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell. 1996;85:171–182. pmid:8612270
- 23. Azpiroz R. An Arabidopsis brassinosteroid-dependent mutant is blocked in cell elongation. Plant Cell. 1998;10:219–230. pmid:9490745
- 24. Choe S, Dilkes BP, Fujioka S, Takatsuto S, Sakurai A, Feldmann K a. The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis. Plant Cell. 1998;10:231–43. pmid:9490746
- 25. Choe S, Tanaka A, Noguchi T, Fujioka S, Takatsuto S, Ross AS, et al. Lesions in the sterol Δ7reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis. Plant J. 2000;21:431–443. pmid:10758495
- 26. Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, et al. The Arabidopsis dwf7/ste1 mutant is defective in the delta7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Plant Cell. American Society of Plant Biologists; 1999;11:207–221.
- 27. Ephritikhine G, Pagant S, Fujioka S, Takatsuto S, Lapous D, Caboche M, et al. The sax1 mutation defines a new locus involved in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana. Plant J. 1999;18:315–320. pmid:10377996
- 28. Hong Z, Ueguchi-Tanaka M, Shimizu-Sato S, Inukai Y, Fujioka S, Shimada Y, et al. Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant J. 2002;32:495–508. pmid:12445121
- 29. Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, et al. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 2012;7:562–578. pmid:22383036
- 30. Wang L, Feng Z, Wang X, Wang X, Zhang X. DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics. 2010;26: 136–138. pmid:19855105
- 31. Wang J, Duncan D, Shi Z, Zhang B. WEB-based GEne SeT AnaLysis Toolkit (WebGestalt): update 2013. Nucleic Acids Res. 2013;41:W77–W83. pmid:23703215
- 32. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 2001;25:402–408. pmid:11846609
- 33. Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009;10:57–63. pmid:19015660
- 34. Lu T, Lu G, Fan D, Zhu C, Li W, Zhao Q, et al. Function annotation of the rice transcriptome at single-nucleotide resolution by RNA-seq. Genome Res. 2010;20:1238–1249. pmid:20627892
- 35. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5:621–628. pmid:18516045
- 36. Miao X, Luo Q. Genome-wide transcriptome analysis between small-tail Han sheep and the Surabaya fur sheep using high-throughput RNA sequencing. Reproduction. 2013;145:587–96. pmid:23579189
- 37. Kovi MR, Zhang Y, Yu S, Yang G, Yan W, Xing Y. Candidacy of a chitin-inducible gibberellin-responsive gene for a major locus affecting plant height in rice that is closely linked to Green Revolution gene sd1. Theor Appl Genet. 2011;123:705–714. pmid:21637999
- 38. Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, et al. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature. 2005;437:693–698. pmid:16193045
- 39. Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, et al. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell. 2003;15:2900–2910. pmid:14615594
- 40. Bishop GJ, Harrison K, Jones JD. The tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family. Plant Cell. 1996;8:959–969. pmid:8672892
- 41. Schultz L, Kerckhoffs LHJ, Klahre U, Yokota T, Reid JB. Molecular characterization of the brassinosteroid-deficient lkb mutant in pea. Plant Mol Biol. 2001;47:491–498. pmid:11669574
- 42. Fujioka S, Choi YH, Takatsuto S, Yokota T, Li J, Chory J, et al. Identification of castasterone, 6-deoxocastasterone, typhasterol and 6-deoxotyphasterol from the shoots of Arabidopsis thaliana. Plant Cell Physiol. 1996;37:1201–1203. pmid:9032971
- 43. Choi YH, Fujioka S, Nomura T, Harada A, Yokota T, Takatsuto S, et al. An alternative brassinolide biosynthetic pathway via late C-6 oxidation. Phytochemistry. 1997;44:609–613.
- 44. Ma Y, Xue H, Zhang L, Zhang F, Ou C, Wang F, et al. Involvement of auxin and brassinosteroid in dwarfism of autotetraploid apple (Malus × domestica). Sci Rep. 2016; 6:26719. pmid:27216878
- 45. Choe S, Dilkes BP, Gregory BD, Ross AS, Yuan H, Noguchi T, et al. The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis. Plant Physiol. 1999;119:897–907. pmid:10069828
- 46. Nomura T, Nakayama M, Reid JB, Takeuchi Y, Yokota T. Blockage of Brassinosteroid Biosynthesis and Sensitivity Causes Dwarfism in Garden Pea. Plant Physiol. 1997;113:31–37. 113/1/31 [pii] pmid:12223591
- 47. Koka CV, Cerny RE, Gardner RG, Noguchi T, Fujioka S, Takatsuto S, et al. A putative role for the tomato genes DUMPY and CURL-3 in brassinosteroid biosynthesis and response. Plant Physiol. 2000;122:85–98. pmid:10631252
- 48. Reid DE, Heckmann AB, Novák O, Kelly S, Stougaard J. CYTOKININ OXIDASE/DEHYDROGENASE3 maintains cytokinin homeostasis during root and nodule development in Lotus japonicus. Plant Physiol. 2016;170:1060–1074. pmid:26644503
- 49. Wasilewska LD, Bralczyk J, Szczegielniak J. The role of gibberellin in regulation of dwarf plants development. Plant Sci. 1987;53:11–19.
- 50. Dubois M, Van den Broeck L, Inzé D. The pivotal role of ethylene in plant growth. Trends in Plant Sci. 2018;23(4):311–323. pmid:29428350
- 51. Vaseva II, Qudeimat E, Potuschak T, Du Y, Genschik P, Vandenbussche F, et al. The plant hormone ethylene restricts Arabidopsis growth via the epidermis. Proc Natl Acad Sci. 2018:201717649. pmid:29643073
- 52. Lee S, Choi SC, An G. Rice SVP-group MADS-box proteins, OsMADS22 and OsMADS55, are negative regulators of brassinosteroid responses. Plant J. 2008;54:93–105. pmid:18182025
- 53. Wang L, Zeng XQ, Zhuang H, Shen YL, Chen H, Wang ZW, et al. Ectopic expression of OsMADS1 caused dwarfism and spikelet alteration in rice. Plant Growth Regul. 2017;81:433–442.
- 54. Kim TW, Michniewicz M, Bergmann DC, Wang ZY. Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway. Nature. 2012;482:419–422. pmid:22307275