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Open Access

Correction

Correction: Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells

  • Bo Xu,
  • Xiaojiao Chen,
  • Zhilei Mao,
  • Minjian Chen,
  • Xiumei Han,
  • Guizhen Du,
  • Xiaoli Ji,
  • Chunxin Chang,
  • Virender K. Rehan,
  • Xinru Wang,
  • Yankai Xia
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In Fig 2b, the image appearing in the GADPH row is incorrect. Please see the complete, correct Fig 2 here.

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Fig 2. Effects of PFOS on pluripotency and expressions of miR-145, miR-490-3p in mESCs.

Cells were cultured with various concentrations of PFOS (0.2 μM, 2 μM, 20 μM, and 200 μM) or DMSO as control for 24 h. (A) Oct-4/Sox-2/Nanog mRNA levels were determined by quantitative real-time PCR using a housekeeping gene GAPDH as an internal control. (B) The protein levels of Oct-4/Sox-2/Nanog were determined by Western blot analysis using GAPDH as an internal control. (C) miRNA levels (miR-145, miR-490-3p) were determined by quantitative real-time PCR and were normalized to U6 as an internal control. Each data point was normalized to the control (DMSO) and represented the means ± S.E. from three independent experiments. (D) Relative protein levels of Oct4, Sox2 and Nanog. *indicates significant difference when the values were compared to that of the control (p<0.05).

https://doi.org/10.1371/journal.pone.0221486.g001

Reference

  1. 1. Xu B, Chen X, Mao Z, Chen M, Han X, Du G, et al. (2013) Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells. PLoS ONE 8(10): e74968. https://doi.org/10.1371/journal.pone.0074968 pmid:24098361

Citation: Xu B, Chen X, Mao Z, Chen M, Han X, Du G, et al. (2019) Correction: Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells. PLoS ONE 14(8): e0221486. https://doi.org/10.1371/journal.pone.0221486

Published: August 16, 2019

Copyright: © 2019 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.