The Urokinase Receptor (uPAR) Facilitates Clearance of Borrelia burgdorferi
Figure 3
Leukocyte migration in uPAR knock-out mice in response to B. burgdorferi infection in vivo.
C57BL/6 WT and uPAR knock-out mice were intradermally injected with 1×106 B. burgdorferi in PBS in the midline of the neck and mice were sacrificed 6 or 32 hours post inoculation. Skin was harvested, formalin fixed and imbedded in paraffin. Five µm-thick sagittal skin sections were processed and H&E, Ly6G and F4/80 stained by routine histological techniques. Control animals injected with PBS alone did not display influx of leukocytes (data not shown). Slides were scored for influx of leukocytes by an independent pathologist who was blinded to the experimental design. Influx was semi-quantitatively scored on a scale from 0–3, with 0 being no, 1 mild, 2 moderate, and 3 being severe diffuse infiltration. Per group and time point 5 five mice were used, error bars represent SEM. Representative sections are depicted in the figure. A p-value<0.05 was considered statistically significant. * indicating p<0,05, i.e. p = 0,0259; NS, not significant, i.e. p = 0,1475.