Dimerization of Hepatitis E Virus Capsid Protein E2s Domain Is Essential for Virus–Host Interaction
Figure 2
Dimerization of E2s in solution was investigated by analytical ultracentrifugation (AUC).
(A) Sedimentation velocity experiment shows that E2s behaves as a single globular species having the sedimentation coefficient of 2.55S and a hydrated friction ratio of 1.30. (B) Sedimentation equilibrium experiment indicates that E2s mainly exists as a dimer with M.W. 30,651±421 Da. The dissociation constant of the E2s dimer, Kd was fitted as 397±283 nM using the self-association model.