A Metazoan/Plant-like Capping Enzyme and Cap Modified Nucleotides in the Unicellular Eukaryote Trichomonas vaginalis
Figure 4
Guanylyltransferase activity recombinant TvCE.
A. Effect of divalent cations Mg2+ (filled circle), Mn2+ (open square) and Ca2+ (filled triangle). B. Effect of competition with cold nucleotides ATP (open square), CTP (filled triangle), UTP (filled square) and GTP (filled circle). C. Enzymatic activity of wild-type (WT) (filled circle) and mutants [R526A (open square), H125S (filled triangle), C126S (filled square), C126S/R526S (open square)] was compared across a range of [α-32P]GTP concentration. Products were resolved on SDS-PAGE and quantified by liquid scintillation.