A Metazoan/Plant-like Capping Enzyme and Cap Modified Nucleotides in the Unicellular Eukaryote Trichomonas vaginalis
Figure 6
Detection of cap 0 and cap 1 modified nucleotides on T. vaginalis mRNA by 2D-TLC analysis.
A. Detection of cap 0 modified nucleotide using in vivo 32P-labeled mRNA. The predicted migration of unmodified ribonucleotides (A, C, G and U) are shown in all panels for reference and left panel also denote modified ribonucleotide standards migration (dotted ovals). P1 = nuclease P1 digestion; TAP = TAP treatment; SpTgs+TAP = SpTgs incubation prior to TAP treatment. B. Detection of cap 1-modified nucleotide. Unlabeled T. vaginalis mRNA either TAP treated (TAP) or mock-treated (TAP-mock) subjected to 5′-end labeling, nuclease P1 digestion and resolution on 2D-TLC.