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Correction: Inflammatory Stimuli Reprogram Macrophage Phagocytosis to Macropinocytosis for the Rapid Elimination of Pathogens

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There is an error in Figure 4. The lower left panel of Figure 4C (Brightfield +IFNγ) was rotated relative to the fluorescent panels. The corrected version of Figure 4 can be seen here.

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Figure 4. Importance of serine phosphorylated coronin 1 for mycobacterial killing and cargo delivery via macropinocytosis.

A–D. Coronin 1-deficient macrophages expressing Cor1-EGFP (A), Cor1S-AEGFP (B) or Cor1S-EEGFP (C), were left untreated or stimulated with IFN-γ followed by infection with M. bovis BCG. Cells were fixed and stained with anti-LAMP1 and anti-mycobacterium antibodies followed by staining with AlexaFluor568- and AlexaFluor647-conjugated secondary antibodies, respectively. Bar: 10 µm. D. Quantitation represents percentage colocalization of bacteria with LAMP1 in cells expressing the indicated constructs (n  =  20; three independent experiments). E,F. Survival of M. bovis BCG in coronin 1-deficient macrophages expressing EGFP alone, Cor1-EGFP, Cor1S-AEGFP and Cor1S-EEGFP respectively, either in non-activated(E) or activated (F) macrophages (mean values ± SD from 3 independent experiments).

https://doi.org/10.1371/journal.ppat.1003879.g004

Reference

  1. 1. BoseDasgupta S, Pieters J (2014) Inflammatory Stimuli Reprogram Macrophage Phagocytosis to Macropinocytosis for the Rapid Elimination of Pathogens. PLoS Pathog 10(1): e1003879