Cryptococcus gattii VGIII Isolates Causing Infections in HIV/AIDS Patients in Southern California: Identification of the Local Environmental Source as Arboreal
Figure 9
C. gattii VGIIIb isolates display higher antifungal susceptibility values to Amphotericin B and flucytosine in contrast to VGIIIa isolates.
(A, E) MIC of VGIIIa (red) versus VGIIIb (blue) for amphotericin B (A), flucytosine (B), fluconazole (C), and ketoconazole (D). MIC of clinical (purple) and environmental (green) isolates of VGIIIa (triangles) and VGIIIb (circles). The MIC of VGIIIa isolates was significantly higher than VGIIIb isolates for amphotericin B (p = 0.0178) and flucytosine (p<0.0001) but not for fluconazole (p = 0.1059) or ketoconazole (p = 0.0685). No significant differences were observed between environmental and clinical isolates of VGIIIa or of VGIIIb in response to amphotericin B (E), flucytosine (F), fluconazole (G), or ketoconazole (H) (Table S10a, S10b and S10c). MIC values, which exceeded the maximum concentration for each Etest, were assigned as the maximum concentration tested for averaging and graphing (Table S1). Maximum concentrations of Etest amphotericin B (32 µg/ml), fluconazole (256 µg/ml), flucytosine (32 µg/ml), and ketoconazole (32 µg/ml).