There is an error in the composition of the TS-solution used for the solubilisation of the tegument contained in the Materials and Methods section. Instead of 0.5 g of each detergent, 0.1 g of each detergent was dissolved in 100 ml PBS. The correct information is as follows: After removal of medium and addition of 500 µl of tegument solubilisation (TS)-solution (0.1 g Brij35 (Roth), 0.1 g Nonidet P40-Substrate (Fluka), 0.1 g Tween80 (Sigma), and 0.1 g TritonX-405 (Sigma) per 100 ml PBS (137 mM NaCl, 2.6 mM KCl, 10 mM Na2HPO4, 1.5 mM KH2PO4 in DEPC-H2O, pH 7.2–7.4)) the reaction vessels were incubated at 37°C and 1,200 rpm in a thermal shaker (TS-100, Biosan) for 5 min to solubilise the tegument in order to make the sub-tegumental musculature accessible for further processing.
Citation: Hahnel S, Lu Z, Wilson RA, Grevelding CG, Quack T (2013) Correction: Whole-Organ Isolation Approach as a Basis for Tissue-Specific Analyses in Schistosoma mansoni. PLoS Negl Trop Dis 7(10): 10.1371/annotation/7aad0212-557d-4121-a72f-da2c115ce534. https://doi.org/10.1371/annotation/7aad0212-557d-4121-a72f-da2c115ce534
Published: October 17, 2013
Copyright: © 2013 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing interests: No competing interests declared.