Death and population dynamics affect mutation rate estimates and evolvability under stress in bacteria
Fig 3
Growth and death dynamics of populations treated with sub-MIC antimicrobials.
Each panel shows data for treatment with a different antimicrobial. Blue dots (left axis) joined by straight blue line represent population sizes measured by plating, expressed as CFUs per mL of culture. Red line (right axis) represents relative death rates, computed from plasmid segregation data, corresponding to the average number of death event per division event between two successive time points. For each treatment, the behaviors of at least 4 fully independent biological replicates, performed on different days with different batches of medium and comprising at least 4 replicate populations, are averaged. Error bars indicate standard error of the mean between biological replicates. Death rates higher than 5 were set to 5. See also S1 Supporting Information for a representation of all biological replicates. Underlying data have been uploaded to Zenodo (10.5281/zenodo.1211765). CFU, colony-forming unit; MIC, minimal inhibitory concentration.
