Death and population dynamics affect mutation rate estimates and evolvability under stress in bacteria
Fig 4
Mutation rate when treating with a sub-MIC dose of kanamycin, H2O2, or norfloxacin compared to untreated.
The uncorrected mutation rate is the one that would be computed based on our data when death is not taken into account. Each point corresponds to a fully independent biological replicate comprising 24 parallel populations. All the computed mutation rates are normalized by the average mutation rate computed in absence of treatment. The horizontal dashed line (y = 1) thus indicates the mutation rate of untreated populations. For each treatment, we performed a paired t test to estimate whether the corrected mutation rate was significantly lower than the uncorrected one (* p < 0.05, ** p < 0.01). Underlying data have been uploaded to Zenodo (10.5281/zenodo.1211765).
