After publication of this article [1], concerns were raised about potential lane similarities and discontinuities in the top left western blot panel in Fig 10F (IP: total α5β1, results for Membrane expression and the 0 timepoint). Specifically:

• Lane 1 appears similar to lane 2.
• When levels are adjusted to visualize background details, there appear to be vertical discontinuities on either side of lane 2.

The first and corresponding authors stated that an error was made in assembling this figure panel and the wrong blot was included. They further stated that they have not been able to recover the original data for the top left western blot panel in question.

The authors provided a corrected version of Fig 10 in which the top left western blot panel was replaced (see below), which they state, includes the correct data from the original experiment. However, the authors have been unable to provide the original data underlying the top left western blot panel that was originally published in Fig 10F, although they have provided all data underlying the remainder of the original Fig 10 panels, as well as those underlying the corrected top left western blot panel in Fig 10F (S1–S3 Files).

The first and corresponding authors stated that the correction of the upper left western blot of panel F of Fig 10 does not change the finding, documented in the original upper right western blot, that silencing of Myosin 6 only modestly influences the endocytosis of total α5β1 integrin in endothelial cells.

PLOS Biology concluded that the results reported for the Fig 10F experiment are adequately supported by the data reported for timepoints 2–20 (upper right blot). However, in light of the unavailability of the underlying data for the original top left western blot in panel F of Fig 10, the concerns about this western blot remain unresolved and so the PLOS Biology Editors issue this Expression of Concern.