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Simulation and Theory of Antibody Binding to Crowded Antigen-Covered Surfaces

Fig 6

A) Plot of the fraction of bivalently bound sites as a function of the apparent (face-value) hapten density. Shaded regions correspond to the prediction of our model, Eq (8), for values of the Fab arm length between 6 and 7 nm (lower and upper curves delimiting the filled regions). Symbols are the experimental data reported in [32]. B) Plot of the fraction of bivalently bound molecules as a function of the rescaled surface density of haptens, . For the numerical data, the values reported in Table 1 were used. For the experimental data from [32], we used the value reported in the paper mol/m². The theoretical prediction (x/(2 + x)) is given by the Langmuir isotherm Eq (12).

doi: https://doi.org/10.1371/journal.pcbi.1004752.g006