Modeling of the axon membrane skeleton structure and implications for its mechanical properties
Fig 2
Young's moduli of rat hippocampal neuronal subcompartments determined by AFM.
Histograms of Young’s moduli of rat hippocampal (A) soma, (B) dendrites, (C) axons, and (D) axons treated with 20μm Latrunculin B. The median Young's modulus of the soma is 0.7 ± 0.2 kPa (A), of dendrites is 2.5 ± 0.7 kPa (B). For the axon plasma membrane, the median Young’s modulus is 4.6 ± 1.5 kPa (C). When axons were treated with Latrunculin B (20μm, 1 hour) the median value of the axon plasma membrane Young’s modulus was reduced to 2.2 ± 0.6 kPa. Number of samples (N = 2), total number of tested neurons (n = 8). N = 1 and n = 6 for axon + Latrunculin B. (E) Box-whisker plots of mean Young's moduli of the soma, dendrites, axon, and axon treated with Latrunculin B. *** indicates statistical significance of p < 0.001 (Kruskal-Wallis test).
