Unraveling ChR2-driven stochastic Ca2+ dynamics in astrocytes: A call for new interventional paradigms
Fig 4
Model sensitivity to light intensity, Ca2+ buffering, and ChR2 parameters.
A) Effect of different light intensities and stimulation paradigms on the basal level and mean spiking rate of a ChETA-expressing astrocyte. Light intensity was varied as a fraction of the control value (I0). Stimulation paradigms were selected based on the 45-min spiking rate heat map in Fig 3A for low (△), medium (○), and high (◊) activity regions. Data is shown as mean ± std (trials = 5). B) Global sensitivity analysis of the astrocytic Ca2+ response to variations in ChR2 and Ca2+ buffering parameters during light stimulation (Δ: T = 2 s, δ = 1% (0.02 s)). Parameters were allowed to vary within a range (Table 2) and 1000 parameter sets were selected using Latin Hypercube Sampling (LHS) with uniform distribution. The partial rank correlation coefficient (PRCC) of each parameter was calculated as a measure of their effect on the basal level (red) and spiking rate (blue) of a ChR2-expressing astrocyte. * denotes statistically significant (p < 0.05) positive or negative influence.
