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Unraveling ChR2-driven stochastic Ca2+ dynamics in astrocytes: A call for new interventional paradigms
Fig 5
Network-wide astrocytic Ca2+ response to light stimulation.
A 10-by-10 network of astrocytes was employed to analyze the response of cells when the central 4-by-4 astrocytes (white square in heatmaps of panels A and C) were stimulated (◊: T = 4 s, δ = 45% (1.8 s), 45 min). See the network organization schematic in Fig 1. Simulations were conducted in the presence (left panel) and absence (right panel) of Ca2+ buffering with varying gap junctional Ca2+ coupling coefficient (
). A symmetric 2D Gaussian fit was utilized to quantify the response, i.e., peak and magnitude of the spread from the stimulated region. A) Heat maps of network-wide light stimulation-induced Ca2+ basal levels. B) Plots of the peak basal level and σbasal-level obtained from the Gaussian fit with varying 
values. Vertical dashed grey line denotes the 
value used to generate the heat maps in panels A and C. C) Heat maps of network-wide spiking rate response corresponding to basal levels in panel A. For spike classification, a threshold of 0.2 μM above the basal level was selected. D) Plots for the peak spiking rate and σspiking rate with varying 
values.
doi: https://doi.org/10.1371/journal.pcbi.1008648.g005
