Genomic and Functional Studies of Drosophila Sex Hierarchy Regulated Gene Expression in Adult Head and Nervous System Tissues
Figure 4
Independent Validation of Microarray Expression Results using RT-PCR
Fold-change (FC) from averaged microarray data for each validated gene is shown at the top of each panel. Each genotype of the target pair compared in the microarray experiment is indicated at either side of the gradient bar. White in the gradient bar represents no difference in gene expression between the two genotypes (FC = 1), and the white to black gradient indicates increasingly higher expression differences in the genotypes indicated. The FC in gene expression between the two genotypes is shown below the gradient bar in the gray-scaled box. At the bottom of each panel are the results of RT-PCR analysis, for which the expression of a given gene relative to rp49 ribosomal expression is reported. Microarray and RT-PCR results for (A) cpn, (B) dpr-family members, and (C) capa. (A) RT-PCR showed that cpn expression relative to rp49 expression was significantly higher (p < 0.015, 3.5-fold) in dsxD female (mean = 9.32, SE = 1.99) than in CS female (mean = 2.64, SE = 0.49) adult heads.
(B) dpr2, dpr7, dpr14, and dpr20 microarray expression, with higher expression in males than fru P1 males. dpr RT-PCR expression relative to rp49 expression was significantly higher (p < 0.05, 4.4-fold) in CS male (mean = 3.47, SE = 0.94) than fru P1 male (mean = 0.78, SE = 0.004) adult heads.
(C) RT-PCR showed that capa expression relative to rp49 expression was significantly higher (p < 9.2E-4, 1.4-fold) in CS male (mean = 0.063, SE = 0.003) than fru P1 male-dissected (mean = 0.047, SE = 0.003) CNS tissue. Error bars represent SE. One-tailed t-test: *, p < 0.05; **, p < 0.001.
doi:0.1371/journal.pgen.0030216.g004
