Genomic and Functional Studies of Drosophila Sex Hierarchy Regulated Gene Expression in Adult Head and Nervous System Tissues
Figure 6
dpr Expression in the CNS Colocalizes with FRUM in Adults and 48-h Pupae and Is Not Sexually Dimorphic
In all images, dpr-GAL4; UAS-nlsGFP (hereafter called dpr expression) is shown in green.
(A–C) dpr expression in the 0–24-h adult male CNS. (A) Anterior brain z-section. (B) Posterior brain z-section. (C) Anterior ventral nerve cord z-section.
(D–F) dpr expression in the 0–24 h adult female CNS. (D) Anterior brain z-section. (E) Posterior brain z-section. (F) Anterior ventral nerve cord z-section.
(G–H) dpr and FRUM (red, immunohistochemistry) expression in the 0–24-h adult male CNS. (G) dpr and FRUM expression in the anterior male brain. Colocalized expression of dpr (green) and FRUM (red) is observed in the median bundle neurons (white box). dpr is expressed in cells in the axonal tract extending from the median bundle (arrow), and in the pars intercerebralis (PI). (H) dpr and FRUM expression in the 0–24-h adult male ventral nerve cord. Colocalized expression of dpr (green) and FRUM (red) is observed in the first thoracic ganglion (white box). (J) dpr (green) and FRUM (red) expression in 40× magnification of median bundle neuron region (white box in [G], independent brain). (K) dpr (green) and FRUM (red) expression in 40× magnification of first thoracic ganglion region (white box in [H], independent VNC). dpr and FRUM expression in the 48-h pupal male brain (I) and ventral nerve cord (L). Z-section thickness was ∼1 μm for (A–I), (L), and ∼0.5 μm for 40× images (J–K).
doi:0.1371/journal.pgen.0030216.g006
