The Use of Orthologous Sequences to Predict the Impact of Amino Acid Substitutions on Protein Function
Figure 1
Example growth curves from which rate metrics were calculated.
Shown are two examples (major MTHFR allele (open triangle); Y189H substitution variant (closed circle)) where growth in liquid culture was tracked over time, according to Methods. The upper panel shows absorbance (OD595) values and the lower panel shows the log10 transformation of the same absorbance reads. Log10-transformed data were used to calculate maximum slopes that served as growth-rate metrics.
