The Use of Orthologous Sequences to Predict the Impact of Amino Acid Substitutions on Protein Function
Figure 2
The average maximum slope (growth-rate metric) and standard deviation for each of the 36 MTHFR variants tested as in Methods. Replicate sets (N = 5) were compared against a positive control (major MTHFR allele) and a negative control (A222V allele) using 2 different statistical criteria as described in Methods. Green circles indicate changes not significantly different from the positive control and significantly better than the A222V control and indicate functionality. Red squares indicate changes significantly less active than the positive control and not significantly better than the A222V control and indicate impaired alleles. Pink triangles are classified as equivocal due to disagreement in the statistical methods. The raw replicate data and statistical metrics are in Table S1.
