Elevated Expression of the Integrin-Associated Protein PINCH Suppresses the Defects of Drosophila melanogaster Muscle Hypercontraction Mutants
Figure 2
Expression of PINCH-Flag fully rescues the larval lethality of the flw7 mutant independently of MRLC/Sqh dephosphorylation.
A–D) For each genotype, graphs show the percentage of animals of the given developmental stage at the indicated time points. Pupae present at the final time point shown in A and C eventually eclosed as adults, whereas pupae in B and D subsequently arrested. E) Representative western blot of four-day-old L3s confirms that PINCH expression is elevated in the PINFL2 and flw7; PINFL2 larval lysates (transgenic PINCH is 44% of endogenous levels for both samples, by densitometric analysis) and that PINFL2 expression does not affect the phosphorylation state of MRLC/Sqh. The ribosomal protein RACK1 is used as a loading control. F) Survival of flw7; stck17/+ larvae (n = 44) is plotted against time.
