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Elevated Expression of the Integrin-Associated Protein PINCH Suppresses the Defects of Drosophila melanogaster Muscle Hypercontraction Mutants

Figure 4

PINCH associates with ILK and RSU1 in order to suppress the larval lethality of flw⁷ mutants.

Survival curves show normal developmental progression upon expression of transgenic PINCH^Q38AFlag (A) or PINCH^D303VFlag (C) alone. Pupae present at day 12 subsequently progress to adulthood. Upon introduction of the mutant PINCH transgenes into a flw⁷ background (B,D), arrest during the larval stages is observed. E) Western blot plus densitometric analysis for PINCH shows the relative level of expression of the mutant Q38A and D303V transgenes as a percentage of endogenous PINCH. The ribosomal protein RACK1 was used as a loading control.

doi: https://doi.org/10.1371/journal.pgen.1003406.g004