NSAIDs Modulate Clonal Evolution in Barrett's Esophagus
Figure 5
Clonal evolution in individuals l and f.
(Panel A) Amount of SGA in biopsies (dots) and mean SGA over all biopsies at that time point (lines) for individual l (black) and individual f (red). (Panels B and C) Circos plots showing genome-wide view of SGA over time (see Figure 4 legend for details). (Panel B) Individual l. During the off-NSAID period we detected a whole-chromosome gain of chromosome 8 in biopsy 12 (green band) and copy-neutral LOH events on chromosome 1 in biopsies 9 and 11 (orange bands). (Panel C) We detected 1,844±573 of SGAs in individual f, who opted for esophagectomy for high-grade dysplasia after 6.4 years of follow-up and died of another cancer 11.9 years later. (Panels D, E, F) BEAST consensus tree, parsimony tree of SGA number, and parsimony tree of SGA quantity for individual l. (Panels G, H, I) BEAST consensus tree, parsimony tree of SGA number, and parsimony tree of SGA quantity for individual f. In both individuals, BEAST trees reveal long-term co-existence of multiple clones. and maximum parsimony trees reveal an underlying progressive evolution of SGA events irrespective of time. Phylogenetic trees generated as indicated in the legend to Figure 4.
