Box plots show the summary phenotype data of the two parents (Bristol in orange and Hawaii in blue), the two nearly isogenic lines (kyIR9 in orange and qgIR1in blue), and the two independent npr-1 loss-of-function alleles (npr-1(ad609) and npr-1(ky13) in red). From left to right, the lifetime fecundity (A), the mean length of adult animals (B), and the LT50 distribution after exposure to S. aureus (C). Below each plot are two boxes. The top box denotes the npr-1 genotype: laboratory-derived NPR-1V from Bristol in orange, ancestral wild-type NPR-1F from Hawaii in blue, or loss-of-function allele from Bristol in red. The bottom box denotes the genome-wide genotype: Bristol in orange and Hawaii in blue. Statistical significance was tested using Tukey's HSD. For (A), the qgIR1 strain has significantly fewer offspring than the Bristol strain (p  =  0.003) does, and the kyIR9 strain has significantly more offspring than the Hawaii strain (p  =  0.0059) does. The Hawaii strain, qgIR1, and the two npr-1 loss-of-function alleles do not have significantly different numbers of offspring. The same is true for the Bristol strain and kyIR9. For (B), the qgIR1 strain is significantly smaller than the Bristol strain (p  =  0.00148), and the kyIR9 strain is significantly larger than the Hawaii strain (p  =  6E-5). The Hawaii strain, qgIR1, and the two npr-1 loss-of-function alleles do not have significantly different lengths. The same is true for the Bristol strain and kyIR9 introgression strain.

https://doi.org/10.1371/journal.pgen.1004156.g003