acal is a Long Non-coding RNA in JNK Signaling in Epithelial Shape Changes during Drosophila Dorsal Closure
Figure 7
acal regulates aop expression.
(A) Genetic interaction between aop and acal mutants. Embryos surviving embryogenesis represent the open space above bars to amount to a hundred percent total embryos analyzed. Number of animals analyzed: aop+/+,acal5/5 = 391, aop1/+,acal5/5 = 253, aop1/1,acal+/+ = 269, aop1/1,acal5/+ = 185. (B) Relative expression of aop in wild type and mutant embryos, as determined by qPCR. (C) Expression of a constitutive active aop transgene (UAS-aopACT) in the ectoderm (69B-gal4) or in the lateral epidermis (pnr-gal4), of wild type or acal mutant embryos. Number of animals analyzed: 69B>aopACT = 642, 69B>aopACT;acal5/5 = 210, pnr>aopACT = 310, pnr>aopACT;acal5/5 = 180. (D) acal over-expression under the 69B-gal4 driver results in significantly decreased Cka and aop expression. In (B) and (D), graphs show mean values +/− SEM of at least three independent experiments run twice.
