Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes
Fig 3
RAB-10 and AMPH-1 contribute to the endosome recruitment of TBC-2.
All images were collected from living intact adult animals expressing intestine-specific transgenes. Representative confocal images of GFP-TBC-2 in wild-type (A), rab-10(ok1494) mutant (B), and amph-1(tm1060) mutant (C) backgrounds are shown. The endosomal localization of GFP-TBC-2 is strongly reduced in rab-10(ok1494) and amph-1(tm1060) mutant backgrounds. (Scale bar: 10 μm) (D) Quantification of the average total intensity of GFP-TBC-2 labeled structures. (n = 18 each, 6 animals of each genotype sampled in three different regions of each intestine.) Error bars represent SEM. ***P<0.001(student's t test). (E) Western Blot analysis of young adult worms expressing GFP-TBC-2 in wild-type, rab-10(ok1494) mutant, and amph-1(tm1060) mutant backgrounds. Blots with anti-GFP and anti-actin antibodies are shown.
