Co-evolution of Bacterial Ribosomal Protein S15 with Diverse mRNA Regulatory Structures
Fig 6
(A) Rate4Site Z-value indicating degree of conservation for each alignment of S15 coding regions. Blue points correspond to values from the Ec-alignment, and green to values from the Gk-alignment. Lower values are more highly conserved positions. Solid arrows indicate positions that are conserved in both the alignments but have different amino acid identities. Open arrows indicate positions that are conserved in one alignment but not in others, red arrows indicate mutation present in Gk-S15-6MUT. (B) Conservation of individual amino acids within each alignment (generated with Weblogo [66]). Residue actually present in the Gk-S15 sequence colored in green, residue actually present in the Ec-S15 sequence colored in blue. (C) Secondary structure diagram of S15, indicating looped or alpha-helix regions, and regions that interact with either the three-way junction (red) or the GU/GC motif of rRNA (green). (D) Regulation of Ec-mRNA and Ec-mRNA-M1. In contrast to Gk-S15, Gk-S15-6MUT regulates Ec-mRNA, and this interaction is abolished in Ec-mRNA-M1. (E) Regulation of Gk-mRNA, Gk-mRNA-M1, and Gk-mRNA-M2. GK-S15-6MUT regulates all three of the Gk-mRNA. Error bars represent standard error for three or more replicates. Data for pEMPTY, and Gk-S15 are repeated from Fig 5 for comparison.
