Next-Generation “-omics” Approaches Reveal a Massive Alteration of Host RNA Metabolism during Bacteriophage Infection of Pseudomonas aeruginosa
Fig 3
PAK_P3 alters expression of many host gene features by late infection.
Differential expression analysis of host gene features comparing transcript abundance between phage negative controls (t = 0 min) and late infection (t = 13min) was performed. This comparison was made after normalizing the read counts that map to each host gene feature between both conditions, ignoring reads that map to the phage, which artificially enriches reads in the late condition. This method thus compares the negative control directly to a host transcript population that has been depleted by replacement with phage transcripts during infection, normalizing away the global depletion so that more specific shifts can be reported and independently tested for.
