Evidence for the evolutionary steps leading to mecA-mediated β-lactam resistance in staphylococci
Fig 3
Alignment of the active centre of PBP2a (highlighted in cyan blue) and representative PBPs putatively encoded by mecA homologues.
The structure of the PBP was predicted by Modeller and the alignment was produced in Pymol. Oxacillin-resistant S. fleurettii 402567 mecA allele 5/PBP2a (A). Oxacillin-susceptible S. vitulinus CH10 mecA allele 2/PBP2a (B). Oxacillin-susceptible S. sciuri K11 mecA1 allele 42/PBP2a.1 (C). Oxacillin-resistant S. sciuri JUG17 mecA1 allele 4/PBP2a (D). 1. Ser401/Ser403. 2. Lys404/Lys406. 3. Tyr444/Tyr446. 4. Ser460/Ser462. 5. Asn464/Asn466. 6. Ser596/Ser598. 7. Thr598/Thr600.
