Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy
Fig 1
Establishment of Ptpn11D61Y mouse strain.
(A) Breeding scheme showing genotypes of parental (F0) and offspring (F1) generation. Progeny segregated in 2 genotypes used as mutants (Ptpn11D61Y) and controls (control) in all experiments. (B) Ptpn11-specific PTP activity was 3-fold higher in Ptpn11D61Y forebrain homogenate as compared to controls. Data are shown as normalized mean ± SEM and analyzed using one sample t-test, *p<0.05. Dots indicate the values of 3 Ptpn11D61Y animals. (C) The expression of Ptpn11 is not altered in the forebrain of Ptpn11D61Y mice. (D) The overall brain morphology is not affected in Ptpn11D61Y animals. Sagittal sections of brains from control and Ptpn11D61Y mice were stained with antibodies against Bsn, VGAT, and VGLUT and with DAPI. Scale bar: 3.5 mm.
