Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy
Fig 4
Synaptic expression and surface trafficking of glutamate receptors is affected in Ptpn11D61Y.
(A) Representative examples of the staining of synaptic surface (left) and total (right) glutamate receptors containing GluA1 or GluN2B subunit in neurons from control and from Ptpn11D61Y. Excitatory synapses were stained for Homer1 and Bassoon. (B, C) Synaptic IF intensity and density (puncta per 20 μm of proximal dendrite) of surface (B) and total (C) staining for all tested receptor subunits are shown. Values are normalized to the respective value in control, represented by the dashed line in the graphs. Note the reduced surface expression of GluA2 and GluN2B and the decrease of the overall expression of GluA1 in Ptpn11D61Y neurons as compared to controls. All numerical values and statistics are listed in S1 Table. (D, E) The rate of internalization of the GluA1-containing AMPARs is reduced in Ptpn11D61Y cells. Scale bars: 5 μm. Data are presented as mean ± SEM and analyzed using unpaired t-test (*p≤0.05, **p≤0.01, ***p≤0.001). Numbers in columns indicate the number of cells analyzed.
