Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy
Fig 7
Time course of nuclear pERK accumulation induced by neuronal activity or BDNF is affected in Ptpn11D61Y neurons.
(A, C) Neurons of both genotypes are stained for pERK at different time points upon 4AP/Bic (A) or BDNF (C) application. DAPI staining was used as nuclear mask. Scale bar: 10 μm. (B, D) Quantification of the nuclear pERK level as exemplified in the staining in A and C. The basal levels of nuclear pERK significantly differ between the genotypes (unpaired t-test, ### p≤0.001). The time course upon stimulation by both stimuli differed between Ptpn11D61Y and control neurons. Data are shown as mean ± SEM and numbers in columns of graphs indicate number of analyzed cells. The stimulation-induced changes were compared to basal pERK levels in each genotype and significance was assessed using one-way ANOVA and Dunnett´s multiple comparison test (**p≤0.01 and ***p≤0.001) and shown in black for controls and in gray for Ptpn11D61Y neurons.
