Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade
Fig 3
Electrophysiological analyses reveal abnormalities in responses to pheromone components of male silkworms with mutations in sex determination genes.
(A) Representative EAGs of wild-type and mutant BmMasc, BmPSI, Bmdsx, and Bmfru male moths in response to hexane (upper panel), 10 μg bombykol (middle panel), and 10 μg bombykal (lower panel). (B) Representative single sensillum recording (SSR) from wild-type and mutant BmMasc, BmPSI, Bmdsx, and Bmfru males in response to hexane (upper panel), 10 μg bombykol (middle panel), and 10 μg bombykal (lower panel). The stimulus was applied for 300 ms, indicated by a red line under the trace. (C and D) Mean responses of male antennae to C) 10 μg of bombykol and D) 10 μg bombykal. The statistical significance between WT (n = 10) and BmMasc (n = 7), BmPSI (n = 5), Bmdsx (n = 8), and Bmfru (n = 11) mutant responses was analyzed with one-way ANOVA. Data are shown as means ± SEM; *, **, and *** represent significant differences at the 0.05, 0.01, and 0.001 levels, respectively, compared with the WT-M. (E and F) Mean responses of neurons in male sensillum trichodea to E) 10 μg of bombykol and F) 10 μg bombykal. The statistical significance between WT (n = 30) and BmMasc (n = 50), BmPSI (n = 44), Bmdsx (n = 30), and Bmfru (n = 76) mutants was analyzed with one-way ANOVA. Data are shown as means ± SEM; ** and *** indicates p < 0.01 and p < 0.001, compared with the WT-M, and n.s. indicates no significance.
