Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade
Fig 4
Expression change of olfactory sensory system genes in sex determination gene mutants.
(A) Relative mRNA expression levels of BmPBP1, BmPBP2, BmPBP3, BmOR1, BmOR2, and BmOR3 in WT and mutant males. Three individual biological replicates were performed with real-time quantitative PCR (qPCR). Plotted are means ± SEM. *, ** and *** represent significant differences at the 0.05, 0.01 and 0.001 levels with one- way ANOVA, comparing each gene was with the corresponding WT-M; n.s. represents not significant. (B) Summary of the expression change of olfactory system genes in each mutant. ‘-’ represents no significant change compare to WT, ‘↓’, ‘↓↓’, ‘↓↓↓’ represent significant decreased (*, **, ***) compare to WT, ‘↑↑’, ‘↑↑↑’ represent significant increased (**, ***) compare to WT.