Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade
Fig 6
BmOR3 mutant male silk moths have nearly normal electrophysiological responses to bombykol but not bombykal.
(A) Representative EAGs from wild-type and BmOR3 mutant male moths in response to hexane (upper panel), 10 μg bombykol (middle panel), and 10 μg bombykal (lower panel). (B and C) Mean responses of WT (n = 9) and BmOR3 mutant (n = 9) male antennae to B) 10 μg of bombykol and C) 10 μg of bombykal. Data are means ± SEM; n.s. indicates no significant difference and ** represents a significant difference at the 0.01 level as determined by Student’s t-test. (D) Representative single sensillum recording (SSR) of wild-type and BmOR3 mutant male moths in response to hexane (upper panel), 10 μg bombykol (middle panel), and 10 μg bombykal (lower panel). The stimulus was applied for 300 ms as indicated with a red line under the trace. (E and F) Mean (± SEM) responses of neurons in male sensillum trichodea to E) 10 μg of bombykol or F) 10 μg of bombykal in WT (n = 26) and BmOR3 mutant (n = 40) male moths. *** indicates p < 0.001 and n.s. indicates no significant difference as determined by Student’s t-test.
