Control of clathrin-mediated endocytosis by NIMA family kinases
Fig 4
The AP2-associated Jowls phenotype is suppressed by nekl–mlt mutants.
(A,B) Representative DIC images of fcho-1(ox477) (A) and fcho-1(ox477); nekl-3(gk506) (B) adults. White arrowheads mark the location of Jowls in fcho-1(ox477) mutants. Bar size in A = 5 μm (for A and B). (C–F) The percentage of adult animals exhibiting the Jowls phenotype was assayed in the indicated genetic backgrounds; “+” indicates that no nekl–mlt mutations were present. (C) Assays were carried out in strong/null nekl–mlt backgrounds using the null fcho-1(ox477) allele. (D) Assays were carried out using the dpy-23/μ null alleles fd261 (in the + background), and fd279 (in the nekl-2(fd81); nekl-3(gk894345) background). (E) Assays were carried out using the apa-2 null alleles fd282 (in the + background) and fd280 (in the nekl-2(fd81); nekl-3(gk894345) background). (F) RNAi was carried out in the indicated backgrounds using injection methods. Error bars indicate 95% confidence intervals. p-Values were determined using Fischer’s exact test where proportions were compared to the corresponding wild-type nekl–mlt allele (+) (C,D,E) or non-injected controls (F); ****p < 0.0001, ***p < 0.001, *p < 0.05. Raw data are available in S1 File.
