Control of clathrin-mediated endocytosis by NIMA family kinases
Fig 5
Clathrin defects in nekl-2; nekl-3 mutants are suppressed by reduced AP2 activity.
(A) Representative confocal images of L2–L3 larvae expressing CRISPR-tagged GFP::CHC-1 within the apical region of the hyp7 epidermal syncytium. GFP::CHC-1 localization is shown for wild-type, nekl-2(fd81); nekl-3(gk894345), nekl-2(fd81); fcho-1(fd131); nekl-3(gk894345), and nekl-2(fd81); dpy-23(fd155) nekl-3(gk894345) strains. Inverted fluorescence images are shown to aid clarity. Bar size in A = 5 μm (for A–D). Background subtraction was performed using the same parameters for all images; minimum and maximum pixel values were kept consistent for all images. (B,C) For individual larvae of the indicated genotypes, the mean GFP::CHC-1 intensity (B) and the percentage of GFP-positive pixels above threshold (C) were determined in the apical region of hyp7. Both the group mean and 95% confidence interval (error bars) are shown. p-Values for compared means were determined using two-tailed Mann-Whitney tests; ****p < 0.0001, **p < 0.01. Raw data are available in S1 File.
