Control of clathrin-mediated endocytosis by NIMA family kinases
Fig 8
Clathrin dynamics are altered in NEKL-depleted adults.
(A–E) Representative time-lapse confocal images of FRAP assays performed using the indicated strains in the presence and absence of auxin (20 h). Images show day-2 adults expressing GFP::CHC-1 within the apical region of the hyp7 epidermal syncytium. Background subtraction was performed using the same parameters for all images; minimum and maximum pixel values were kept consistent for all images. Inverted fluorescence was used to aid clarity. Each panel series contains images at the pre-bleach stage together with images at 0 s, 15 s, 30 s, 90 s, and 180 s after bleaching. Red dashed circles indicate the region of photobleaching. Bar size in A = 5 μm (for A–E). (F–H) Corresponding fluorescence recovery curves of GFP::CHC-1 after photobleaching. Normalized average mean intensities of the photobleached regions were plotted as a function of time using 5-s intervals; error bars denote SEM. (F) Fluorescence recovery curves for wild-type, NEKL-2::AID, and NEKL-3::AID adults in the absence of auxin. (G,H) Fluorescence recovery curves for NEKL-2::AID (G) and NEKL-3::AID (H) strains in the presence and absence of auxin. Raw data are available in S1 File.
