Control of clathrin-mediated endocytosis by NIMA family kinases
Fig 10
Cargo trafficking is disrupted in NEKL-depleted adults.
(A,C) Representative confocal images of day-2 adults expressing LRP-1::GFP in the apical region of the hyp7 epidermal syncytium. Background subtraction was performed using the same parameters for all images; minimum and maximum pixel values were kept consistent for all images. Inverted fluorescence was used to aid clarity. Bar sizes in A,C = 5 μm. (B,D) The percentage of GFP-positive pixels above threshold was determined for individual auxin-treated (20 h) wild type and NEKL-3::AID adults (B) and untreated wild-type, ncap-1(mew39), and dpy-23(mew74) (C) adults. The group mean and 95% confidence interval (error bars) are shown. p-Values were determined using two-tailed Mann-Whitney tests; ****p < 0.0001, **p < 0.01. Raw data are available in S1 File.
