Control of clathrin-mediated endocytosis by NIMA family kinases
Fig 12
The human orthologs of nekl-3, NEK6 and NEK7, rescue molting and trafficking defects.
(A,B) Bar plots showing rescue of molting defects in nekl-3(gk506) (A) and NEKL-3::AID (B) strains with the indicated transgenes. NEK6::GFP and NEK7::GFP refer to Pnekl-3::NEK6::GFP and Pnekl-3::NEK7::GFP, respectively. NEK6 and NEK7 refer to Pnekl-3::NEK6 and Pnekl-3::NEK7, respectively. p-Values were determined using Fischer’s exact test; ****p < 0.0001. (C,D) For individual adults, the mean GFP::CHC-1 intensity (left graphs) and the percentage of GFP-positive pixels above threshold (right graphs) were determined for animals expressing wild-type nekl-3 (C) or NEK6 (D). The group mean and 95% confidence interval (error bars) are shown. p-Values were determined using two-tailed Mann-Whitney tests; *p < 0.05, **p < 0.01, ***p < 0.001. (E,F) Fold-changes (ratios) of auxin-treated (20 h) versus untreated day-2 NEKL-3::AID adults expressing the indicated transgenes were determined for apical hyp7 GFP::CHC-1 mean intensities (E) and the percentage of GFP-positive pixels above threshold (F). Error bars indicate 95% confidence intervals. The dashed red line at 1.0 indicates no change in auxin-treated versus untreated worms. Statistical analyses for ratios were carried out as described in the Materials and Methods; **p < 0.01, ***p < 0.001, ****p < 0.0001. Raw data are available in S1 File.
