Replication of the Salmonella Genomic Island 1 (SGI1) triggered by helper IncC conjugative plasmids promotes incompatibility and plasmid loss
Fig 1
Monitoring of pVCR94 and SGI1 in cells by flow cytometry (FC).
A. Schematic representation of chromosomally integrated SGI1. ORFs are represented by gray arrows. Gene numbers correspond to the last digits of locus tags S0xx in the Genbank accession number AF261825. ORFs of interest in this work are shown in brown. AcaCD binding sites are depicted by green angled arrows. Left (attL) and right (attR) junctions with the chromosome are indicated by black bars at the ends. The position of the complex class 1 integron In104 (multidrug resistance region) is indicated by a black arrowhead. B. Fluorescent reporter genes mNeonGreen (green arrow) and mCherry (red arrow) under the control of the PBAD promoter were inserted in pVCR94 between traGC and eexC, and in SGI1 between S009 and S010. C. Representative flow cytometric scatter plots of green and red fluorescence of E. coli KH95 bearing either pVCR94GreenKn or SGI1RedKn. D. Schematic representation of the labelled elements in the cells. SGI1RedKn is integrated into the chromosome at the 3’ end of trmE.
