Replication of the Salmonella Genomic Island 1 (SGI1) triggered by helper IncC conjugative plasmids promotes incompatibility and plasmid loss
Fig 2
Incompatibility between SGI1 and IncC plasmids is trackable by FC.
A. Evolution of the percentage of E. coli KH95 cells bearing SGI1RedKn (SGI1) and pVCR94Green (IncC) and grown over 54 generations in the absence of antibiotics as monitored using FC. Plots represent the mean and standard error of the mean obtained from three independent experiments. ATB is a recovery control culture at G54 in LB with selective pressure for both elements. B. Representative FC density plots of the data presented in panel A mapping the green signal (513 nm) as a function of the red signal (610 nm). C. Representative FC density plots of fluorescence intensity over forward scatter corresponding to data presented in panel B. Color keys are identical in all panels. In panel C, pink and red indicate cells producing low- and high-intensity red fluorescence, respectively.
