Replication of the Salmonella Genomic Island 1 (SGI1) triggered by helper IncC conjugative plasmids promotes incompatibility and plasmid loss
Fig 5
Impact of SGI1 replication on incompatibility.
Evolution of the percentage of E. coli KH95 cells bearing either pVCR94GreenSp (IncC) and pSGI1 (A) or pVCR94Green (IncC) and the ΔtraNS (B), Δint (C), Δxis (D), Δrep (E), or ΔoriV (F) mutants of SGI1Red (SGI1) over 54 generations in the absence of antibiotics as monitored by FC. Inserts for panels C, D and E show complementation experiments using the designated expression plasmid and pVCR94GreenSp. Conditions were identical to Fig 2A. Plots represent the mean and standard error of the mean obtained from three independent experiments. Representative density plots of mNeongreen intensity over mCherry intensity and their corresponding plots of fluorescence intensity over forward scatter are shown for each condition in S1–S5 and S7 Figs.
