Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
Figure 3
SmZF1 heterologously expressed in COS-7 cells is able to bind to its target oligonucleotide.
For EMSA experiments, SmZF1 target oligonucleotide D1-3DNA was incubated with no extract (lane 1) or total extracts of COS-7 cells expressing the proteins YFP (lanes 2–4) or YFP-SmZF1 (lanes 5–7). For supershift reactions, the mixture DNA/extracts was pre-incubated with anti-GFP (lanes 3 and 6) or anti-SmZF1 antibodies (lanes 4 and 7). As negative controls, the anti-GFP and anti-SmZF1 antibodies were also incubated with the oligonucleotide in the absence of cell extracts (lanes 8 and 9, respectively). The resulting 4% PAGE was stained with VISTRA Green DNA specific dye for visualization of bands.
