Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
Figure 4
SmZF1 is able to increase luciferase gene expression in COS-7 cells.
The plasmids of interest plus the TK-Renilla reporter plasmid were transfected into COS-7 cells using the LipofectamineTM 2000 Transfection Reagent. Each transfection was performed in triplicate, and transfection efficiency was normalized to TK-Renilla luciferase reporter plasmid. Luciferase activity was assayed in cell lysates using a TD20/20 luminometer (Promega) and is indicated as Relative Light Units (RLU). Statistical analysis of the data was carried out with Minitab Version 1.4 using Student's t test, and p values<0.05 were considered as significant. Data is representative of three independent experiments.
