Inhibitors of Trypanosoma cruzi Sir2 related protein 1 as potential drugs against Chagas disease
Fig 5
In vitro hepatocytes and neurons injury scores for BNIPSpd (9) by HCS.
The score was calculated as the sum of individual scores obtained from a panel of in vitro cytotoxicity assays that include: mitochondrial dysfunction measured by TMRM probe dynamics in cells; membrane integrity assayed by lactate dehydrogenase quantification; DNA damage by imaging with H2AX antibody; apoptosis by caspase 3/7 activation; and neurite outgrowth as imaged with an anti-tubulin III antibody. Nimesulide (400 μM), an approved drug with a mild toxicological profile, was included as a toxicity control.
