Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies
Fig 1
Performance characteristics of the three LAMP assays.
(A) LAMP targeting the polA gene. All TP strains become positive. (B) LAMP assay targeting the tprL locus, which results in amplification of TPA and TEN strains. (C) Only TPE and TEN strains generate positive results in the LAMP assay targeting the TP_0619 locus. Note that the strain material in the three assays had different TP copy numbers as indicated in S1 Table. Data points are presented as mean±SEM values. Red cross = Negative control; TPA strains (blue) up-pointing triangle = Mexico A, down-pointing triangle = SS14, circle = Nichols, square = Seattle 81–4; TPE strains (green) square = Gauthier, square turned = Sei Geringing K403, hexagon = Kampung Dalan K363, circle = Samoa D, circle with cross = CDC-1, square with cross CDC-2; TPE simian strains (purple) cross = Fribourg-Blanc, star = RNP; TEN strains (black) square = Bosnia A, circle with cross = Iraq B.
